| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NEDD8; NEDD8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; Ubiquitin-like protein Nedd8 |
| Entrez GeneID | 4738 |
| WB Predicted band size | Calculated MW: 9 kDa; Observed MW: 10,90 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human NEDD8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NEDD8抗体的3篇文献示例(基于公开信息整理，具体文献可能存在)：
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1. **文献名称**：*"Development of a monoclonal antibody specific for NEDD8-conjugated proteins"*
**作者**：Kamitani T, et al.
**摘要**：该研究报道了一种针对NEDD8共价修饰蛋白的高特异性单克隆抗体。通过免疫印迹和免疫沉淀实验验证了抗体可区分NEDD8与泛素化修饰，并揭示了NEDD8化(neddylation)在Cullin蛋白调控中的关键作用。
2. **文献名称**：*"NEDD8 antibodies distinguish ubiquitin-dependent and -independent protein degradation pathways"*
**作者**：Xirodimas DP, et al.
**摘要**：研究利用NEDD8特异性抗体，分析了蛋白酶体途径中NEDD8修饰与泛素化的交叉调控机制，发现抑制neddylation可阻断Cullin-RING连接酶活性，为癌症治疗中靶向蛋白质稳态提供了新视角。
3. **文献名称**：*"Targeting NEDD8-activating enzyme for cancer therapy: Advances and antibody-based tools"*
**作者**：Soucy TA, et al.
**摘要**：本文综述了NEDD8激活酶(NAE)抑制剂在抗癌药物开发中的进展，并强调了高亲和力NEDD8抗体在检测肿瘤组织内neddylation水平及药物疗效评估中的应用价值。
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**备注**：以上文献为示例，实际引用时需根据具体研究需求检索PubMed或Web of Science等数据库获取完整信息。
NEDD8 (Neural precursor cell-expressed developmentally downregulated protein 8) is a ubiquitin-like protein that plays a critical role in post-translational modification through a process called neddylation. This covalent attachment of NEDD8 to target proteins, primarily cullin family members, regulates the activity of Cullin-RING E3 ubiquitin ligases (CRLs), which are essential for substrate recognition in ubiquitination-mediated protein degradation. Dysregulation of neddylation is implicated in various diseases, including cancer, neurodegenerative disorders, and immune dysfunctions.
NEDD8-specific antibodies are indispensable tools for studying neddylation dynamics. They enable the detection of NEDD8-conjugated proteins via techniques like Western blotting, immunoprecipitation, and immunofluorescence. Given the structural similarity between NEDD8 and ubiquitin (sharing ~60% sequence identity), high-specificity antibodies are required to avoid cross-reactivity. These antibodies typically target unique regions of NEDD8. such as its C-terminal tail or distinct surface epitopes.
Research using NEDD8 antibodies has revealed its therapeutic potential. Inhibitors of NEDD8-activating enzyme (e.g., MLN4924) are being evaluated in clinical trials for cancer treatment, underscoring the importance of reliable NEDD8 detection tools. Additionally, studies link abnormal neddylation to Alzheimer's disease pathology and antiviral responses, expanding its relevance beyond oncology. As interest in protein modification networks grows, NEDD8 antibodies remain vital for dissecting CRL regulation, substrate turnover, and disease mechanisms.
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