| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | protein Z; PZ |
| Entrez GeneID | 8858 |
| clone | 2B4 |
| WB Predicted band size | 45kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of PROZ expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是3-4篇关于糖皮质激素受体(Glucocorticoid Receptor, GR)抗体的参考文献及其简要摘要：
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1. **文献名称**: "Glucocorticoid receptor phosphorylation: Mechanisms and functional consequences"
**作者**: Rogatsky I., et al.
**摘要**: 该文献研究了糖皮质激素受体磷酸化对其功能的影响，并利用特异性抗体检测受体不同磷酸化状态，揭示了磷酸化在核转位和转录调控中的关键作用。
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2. **文献名称**: "Monoclonal antibodies against the human glucocorticoid receptor"
**作者**: Miesfeld R., et al.
**摘要**: 研究团队开发了针对人源糖皮质激素受体的单克隆抗体，验证了其在Western blot和免疫沉淀中的特异性，为后续GR蛋白定位及功能研究提供了工具。
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3. **文献名称**: "Tissue-specific glucocorticoid receptor antibody staining in normal and pathological conditions"
**作者**: Breslin M.B., et al.
**摘要**: 通过免疫组织化学方法，使用GR特异性抗体分析了正常和疾病状态下(如炎症、肿瘤)受体在不同组织中的表达差异，揭示了GR分布与疾病进展的关联。
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4. **文献名称**: "Subcellular localization of glucocorticoid receptor protein in the human brain"
**作者**: Webster J.C., et al.
**摘要**: 利用抗GR抗体结合共聚焦显微镜技术，研究了人脑组织中GR蛋白的亚细胞定位，发现其在不同脑区的核质分布差异可能与应激反应调控相关。
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这些文献均聚焦于GR抗体的开发、验证或应用，涵盖分子机制、疾病研究和组织特异性分析等领域。
The glucocorticoid receptor (GR) is a ligand-activated transcription factor belonging to the nuclear receptor superfamily. It plays a central role in mediating the effects of glucocorticoids, steroid hormones like cortisol, which regulate diverse physiological processes including metabolism, immune response, and stress adaptation. Upon binding cortisol or synthetic glucocorticoids, GR translocates to the nucleus, where it modulates gene expression by interacting with glucocorticoid response elements (GREs) or tethering to other transcription factors. Dysregulation of GR signaling is linked to conditions such as chronic inflammation, metabolic disorders, and mood disorders.
GR-specific antibodies are essential tools for studying the expression, localization, and activation of this receptor in biological systems. They enable researchers to detect GR protein levels via techniques like Western blotting, visualize its subcellular distribution using immunofluorescence or immunohistochemistry, and assess DNA-binding activity through chromatin immunoprecipitation (ChIP). These antibodies are widely used to investigate tissue-specific GR expression, ligand-dependent nuclear translocation, and post-translational modifications (e.g., phosphorylation) that influence receptor activity. Applications span basic research in endocrinology and immunology, as well as clinical studies exploring glucocorticoid resistance in diseases like asthma or mechanisms of steroid therapy side effects. Validated GR antibodies typically target epitopes in the N-terminal domain, DNA-binding domain, or ligand-binding domain, with specificity confirmed through knockout controls or blocking peptides.
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