| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AW212142; dual specificity mitogen activated protein kinase kinase 3; Dual specificity mitogen activated protein kinase kinase 6; Dual specificity mitogen-activated protein kinase kinase 3; MAP kinase kinase 3; MAP kinase kinase 6; map2k3; MAP2K6; MAPK ERK kinase 3; MAPK/ERK kinase 3; MAPK/ERK kinase 6; MAPKK 3; MAPKK 6; MAPKK3; MAPKK6; MEK 3; MEK 6; MEK3; Mitogen activated protein kinase kinase 3; Mitogen activated protein kinase kinase 6; MKK 3; MKK3; MKK6; mMKK3b; MP2K3_HUMAN; PRKMK 3; PRKMK3; PRKMK6; Protein kinase; mitogen activated; kinase 6 (MAP kinase kinase 6); protein kinase; mitogen-activated; kinase 3; SAPK kinase 2; SAPKK 2; SAPKK 3; SAPKK-2; SAPKK2; SAPKK3; Stress activated protein kinase kinase 2; Stress activated protein kinase kinase 3; Stress-activated protein kinase kinase 2. |
| Entrez GeneID | 5606 |
| WB Predicted band size | Calculated MW: 39 kDa; Observed MW: 39 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human MEK3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MEK3抗体的3篇参考文献及其摘要内容：
1. **"Role of MEK3 in p38 MAPK Activation During Inflammatory Responses"**
*作者：Han, J. et al.*
摘要：本研究利用MEK3特异性抗体，通过Western blot和免疫沉淀技术，证实MEK3在炎症因子刺激下磷酸化并激活下游p38 MAPK通路，揭示了其在巨噬细胞炎症反应中的关键作用。
2. **"Characterization of a Monoclonal Antibody Against MEK3 for Signal Transduction Studies"**
*作者：Smith, L.M. & Johnson, R.*
摘要：文章报道了一种高特异性MEK3单克隆抗体的开发，验证了其在免疫组化、流式细胞术中的适用性，并证明其可区分MEK3与其他MAP2K家族成员(如MEK2/MEK6)，为通路研究提供可靠工具。
3. **"MEK3 Expression and Activity in Tumor Microenvironment Regulation"**
*作者：Lee, Y. et al.*
摘要：通过MEK3抗体分析癌症组织样本，发现MEK3在肿瘤相关成纤维细胞中高表达，并通过调控IL-6分泌促进肿瘤侵袭，提示其作为潜在治疗靶点的价值。
4. **"Antibody-Based Detection of MEK3 Phosphorylation in Cellular Stress Models"**
*作者：Garcia, A. et al.*
摘要：该文献建立了基于MEK3抗体的磷酸化检测方法，证明氧化应激和紫外线辐射可通过不同机制激活MEK3.为研究细胞应激信号提供了方法学支持。
The MEK3 antibody is a crucial tool in studying the mitogen-activated protein kinase (MAPK) signaling pathway, specifically targeting MAP kinase kinase 3 (MAP2K3 or MEK3). MEK3 is a dual-specificity kinase that phosphorylates and activates p38 MAPK, a key regulator of cellular responses to stress, cytokines, and growth factors. This pathway influences processes like inflammation, apoptosis, differentiation, and immune regulation. MEK3 antibodies are widely used in research to detect MEK3 protein expression, assess its phosphorylation status, and investigate its role in diseases such as cancer, neurodegenerative disorders, and autoimmune conditions.
These antibodies are typically developed in hosts like rabbits or mice using immunogenic peptides derived from conserved regions of human MEK3. They are validated for applications including Western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry. Specificity is confirmed using knockout cell lines or siRNA-mediated MEK3 knockdown. Researchers also employ MEK3 antibodies to study cross-talk with other signaling pathways, such as ERK or JNK, and to evaluate pharmacological inhibitors targeting the p38 pathway.
Commercial MEK3 antibodies often include monoclonal and polyclonal variants, with clones like D1A5G (Cell Signaling Technology) being widely cited. Proper controls, such as recombinant protein blocking or isotype-matched antibodies, are essential to ensure accuracy. As dysregulation of MEK3-p38 signaling is implicated in numerous pathologies, these antibodies remain vital for both basic research and drug discovery efforts.
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