| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CCN1; CCNA; CCNA1; CCNA2; CT146; Cyclin-A; Cyclin-A1; Cyclin-A2 |
| Entrez GeneID | 890/8900 |
| WB Predicted band size | Calculated MW: 52,49 kDa; Observed MW: 52,49 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Cyclin A1/A2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Cyclin A1/A2抗体的参考文献示例(注：以下为模拟内容，实际文献需通过学术数据库检索获取)：
1. **文献名称**: "Cyclin A1 expression regulates proliferation and apoptosis in leukemia cells via Bcl-2 modulation"
**作者**: Müller-Tidow C, et al.
**摘要**: 该研究利用Cyclin A1特异性抗体，发现其在急性髓系白血病中过表达，并通过调控Bcl-2家族蛋白促进细胞增殖和抑制凋亡，提示其作为白血病治疗靶点的潜力。
2. **文献名称**: "Distinct roles of Cyclin A2 in somatic cell cycle progression revealed by antibody-mediated knockdown"
**作者**: Pagano M, et al.
**摘要**: 通过Cyclin A2抗体干扰实验，证明Cyclin A2在G1/S期转换和DNA复制中不可或缺，且其缺失导致细胞周期停滞，强调了其在体细胞分裂中的核心作用。
3. **文献名称**: "Cyclin A1/A2 antibody-based profiling identifies tumor-specific expression patterns in solid tumors"
**作者**: Li J, et al.
**摘要**: 研究采用Cyclin A1和A2的双重抗体免疫组化分析，发现Cyclin A1在睾丸癌和乳腺癌中特异性高表达，而Cyclin A2在多种癌组织中普遍上调，可作为癌症分型的辅助标志物。
4. **文献名称**: "Antibody validation for Cyclin A1 in murine germ cell development"
**作者**: Ravnik SE, Wolgemuth DJ
**摘要**: 通过验证Cyclin A1抗体在小鼠模型中的特异性，证实其在精母细胞减数分裂过程中的阶段性表达，为生殖细胞发育机制研究提供了可靠工具。
**建议**：实际文献可通过PubMed/Google Scholar以关键词“Cyclin A1 antibody”、“Cyclin A2 antibody” + “expression”、“function”或具体疾病名(如“cancer”)检索，并筛选涉及抗体应用的实验研究(如Western blot、IHC等)。
Cyclin A1 and Cyclin A2 are key regulatory proteins in the cell cycle, belonging to the cyclin family that drives progression through specific phases by binding and activating cyclin-dependent kinases (CDKs). Cyclin A1 (CCNA1) is primarily expressed in germ cells and exhibits restricted tissue distribution, though its dysregulation is linked to cancers, particularly leukemia and solid tumors. Cyclin A2 (CCNA2), the more ubiquitously expressed isoform, is essential for both S phase (DNA replication) and G2/M transition (mitosis) in somatic cells. Both cyclins interact with CDK1/2 to regulate DNA synthesis, checkpoint control, and chromosomal segregation.
Antibodies targeting Cyclin A1/A2 are critical tools for studying cell cycle dynamics, protein localization, and expression patterns in normal and diseased tissues. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess cell proliferation, tumorigenesis, or response to therapeutics. Specificity is crucial due to the high sequence homology (~60%) between Cyclin A1 and A2. requiring validation to avoid cross-reactivity. Researchers often employ these antibodies to explore associations between cyclin overexpression and cancer prognosis, drug resistance, or genomic instability. Additionally, they help elucidate roles in non-cell cycle processes, such as transcriptional regulation or apoptosis. Proper controls (e.g., knockout cell lines) are recommended to confirm antibody specificity, particularly in overlapping expression contexts like testicular or cancer samples.
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