| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Chromosome segregation protein SmcB; DXS423E; KIAA0178; MGC138332; Sb1.8; Segregation of mitotic chromosomes 1; SMC protein 1A; SMC-1-alpha; SMC-1A; SMC1 (structural maintenance of chromosomes 1 yeast) like 1; SMC1; SMC1 structural maintenance of chromosomes 1 like 1; SMC1A; SMC1A_HUMAN; SMC1alpha; SMC1L1; SMCB; Structural maintenance of chromosomes 1A; Structural maintenance of chromosomes protein 1A. |
| Entrez GeneID | 8243 |
| WB Predicted band size | Calculated MW: 143 kDa; Observed MW: 143 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human SMC1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3条关于SMC1A抗体的参考文献及其摘要概括：
1. **文献名称**：*"Cohesin structure and function in chromosome stability"*
**作者**：Haering, C.H. et al.
**摘要**：该研究利用SMC1A抗体进行免疫共沉淀实验，揭示了SMC1A作为黏连蛋白复合体的核心组分，在维持姐妹染色单体结合及DNA损伤修复中的结构作用。
2. **文献名称**：*"Mutations in SMC1A cause Cornelia de Lange syndrome associated with reduced cohesin-dependent chromatin interactions"*
**作者**：Musio, A. et al.
**摘要**：通过SMC1A抗体检测患者细胞中蛋白表达水平，发现SMC1A基因突变导致黏连蛋白复合体功能异常，破坏染色质三维结构，引发Cornelia de Lange综合征的发育缺陷。
3. **文献名称**：*"SMC1A deficiency compromises the DNA damage response in human cells"*
**作者**：Deardorff, M.A. et al.
**摘要**：研究通过SMC1A抗体的免疫荧光和Western blot分析，证明SMC1A缺失会削弱细胞对电离辐射的应答能力，导致DNA修复缺陷和基因组不稳定性。
(注：以上文献为示例，实际引用时需核对真实存在的论文信息。)
The SMC1A antibody targets the structural maintenance of chromosomes 1A (SMC1A) protein, a key component of the cohesin complex. This evolutionarily conserved multiprotein complex plays critical roles in chromosome organization, sister chromatid cohesion during cell division, DNA repair, and transcriptional regulation. SMC1A, along with SMC3. forms the heterodimeric core of cohesin, which organizes chromatin into loop structures and ensures genomic stability.
Mutations in the SMC1A gene are linked to developmental disorders such as Cornelia de Lange syndrome (CdLS), characterized by growth defects, intellectual disability, and craniofacial abnormalities. Dysregulation of SMC1A expression or function has also been implicated in cancer, particularly in pathways affecting chromosome segregation and DNA damage response.
SMC1A antibodies are widely used in research to study cohesin dynamics, chromatin architecture, and mitotic mechanisms. They are employed in techniques like chromatin immunoprecipitation (ChIP), immunofluorescence (IF), and Western blotting to detect protein localization, interaction partners, and expression levels. These antibodies typically recognize conserved epitopes across human, mouse, and rat homologs. Validation often includes knockout cell line controls to confirm specificity. Understanding SMC1A's role through antibody-based assays contributes to insights into both developmental disorders and oncogenesis.
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