| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Histone H2B type 1-H; Histone H2B.j; H2B/j; HIST1H2BH; H2BFJ |
| Entrez GeneID | 85236 |
| WB Predicted band size | Calculated MW: 14 kDa; Observed MW: 14 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Histone H2B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Histone H2B抗体的3篇参考文献的简要整理：
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1. **文献名称**: "A conserved modification in the N-terminal tail of histone H2B in yeast is critical for chromatin dynamics"
**作者**: Briggs SD, et al.
**摘要**: 研究通过特异性H2B抗体发现酵母组蛋白H2B的N端赖氨酸修饰(如泛素化)在染色质重塑和转录调控中起关键作用，揭示了H2B修饰对异染色质稳定性和基因沉默的影响。
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2. **文献名称**: "Histone H2B monoubiquitination regulates nucleosome dynamics as revealed by single-molecule imaging"
**作者**: Fierz B, et al.
**摘要**: 利用H2B特异性抗体结合单分子成像技术，发现H2B单泛素化修饰通过调控核小体结构动态影响染色质可及性，进而调节DNA复制和修复过程。
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3. **文献名称**: "Antibody-based detection of histone H2B phosphorylation in apoptosis"
**作者**: Cheung WL, et al.
**摘要**: 开发了一种针对H2B磷酸化位点(如Ser14)的特异性抗体，用于检测细胞凋亡过程中组蛋白H2B的磷酸化变化，证实其与DNA断裂和凋亡信号通路的关联。
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4. **文献名称**: "Cross-talk between histone H2B ubiquitination and H3 methylation in gene silencing"
**作者**: Sun ZW, et al.
**摘要**: 通过H2B抗体和ChIP-seq技术，揭示了H2B泛素化与H3K4甲基化之间的相互作用机制，证明两者协同调控基因沉默和端粒区域异染色质的形成。
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这些文献涵盖了H2B抗体在修饰研究、染色质动态、细胞凋亡检测及表观遗传调控中的应用。
Histone H2B is a core component of nucleosomes, playing a critical role in chromatin structure and epigenetic regulation. As one of the five major histone families (H1. H2A, H2B, H3. H4), H2B forms an octamer with histones H2A, H3. and H4. around which DNA wraps to maintain genomic organization. Beyond structural functions, H2B participates in DNA repair, replication, and transcriptional regulation through post-translational modifications (e.g., acetylation, ubiquitination, phosphorylation) that influence chromatin accessibility.
Antibodies targeting Histone H2B are essential tools for studying chromatin dynamics and gene expression mechanisms. They are widely used in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate nucleosome assembly, histone modification patterns, and their roles in cellular processes such as apoptosis or cell cycle progression. Specific H2B antibodies may recognize conserved epitopes across species (human, mouse, rat) or distinguish modified forms (e.g., H2Bub1 for ubiquitinated H2B), enabling research into disease-associated epigenetic changes, including cancer and neurodegenerative disorders. Quality validation often involves knockout controls or peptide competition assays to confirm specificity. These antibodies have become indispensable for exploring chromatin remodeling, histone exchange, and crosstalk between histone modifications in both basic and translational research.
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