| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MEK6; MKK6; MAPKK6; PRKMK6; SAPKK3; MAP2K6; MEK3; MAP kinase kinase 3; MAPKK3; MAPK/ERK kinase 3 |
| Entrez GeneID | 5606/5608 |
| WB Predicted band size | Calculated MW: 39,37 kDa; Observed MW: 39,37 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human MEK3/MEK6 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于MEK3/MEK6抗体的典型参考文献(内容为模拟概括)：
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1. **文献名称**: "Selective activation of the p38 MAPK pathway by engineered MEK3/MEK6 chimeras"
**作者**: Cuenda A, et al.
**摘要**: 该研究通过构建MEK3/MEK6嵌合体蛋白，验证了特异性抗体的应用(Western blot和免疫沉淀)，揭示了MEK3/MEK6在p38 MAPK信号通路中的差异激活机制，为炎症反应研究提供工具支持。
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2. **文献名称**: "MEK6 overexpression drives melanoma progression via ERK-independent signaling"
**作者**: Janeckova L, et al.
**摘要**: 利用MEK6特异性抗体(货号ab12345)分析黑色素瘤组织样本，发现MEK6高表达通过非经典ERK通路促进肿瘤侵袭，提示其作为潜在治疗靶点。
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3. **文献名称**: "MEK3/MEK6 double knockout mice exhibit embryonic lethality due to placental defects"
**作者**: Yang J, et al.
**摘要**: 通过基因敲除模型结合MEK3/MEK6抗体验证(免疫组化)，证明两者协同调控胎盘血管生成，缺失导致胚胎致死，强调了其在发育中的必要性。
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**备注**：以上文献信息为示例，实际引用时需通过PubMed或学术数据库(如PMID/DOI)核对原文准确性。
MEK3 (MAP2K3) and MEK6 (MAP2K6) are dual-specificity protein kinases belonging to the mitogen-activated protein kinase kinase (MAP2K) family. They function as critical upstream activators of the p38 MAP kinase pathway, which regulates cellular responses to stress, inflammation, apoptosis, and differentiation. MEK3 and MEK6 share structural similarities, including a conserved kinase domain, but exhibit distinct substrate preferences and tissue distribution. MEK3 is primarily activated by cytokines and environmental stressors, while MEK6 responds broadly to diverse stimuli, including osmotic stress and pro-inflammatory signals. Both kinases phosphorylate p38 MAPK at Thr180 and Tyr182. triggering its activation and downstream signaling.
Antibodies targeting MEK3/MEK6 are essential tools for studying their expression, phosphorylation status, and interaction partners in various biological contexts. These antibodies enable researchers to investigate their roles in diseases such as cancer, neurodegenerative disorders, and autoimmune conditions, where dysregulated p38 signaling is implicated. Specificity validation via knockout controls is critical due to high homology among MAP2K family members. Applications include Western blotting, immunohistochemistry, and immunofluorescence to map spatial-temporal activation patterns. Recent studies also utilize these antibodies to explore therapeutic targeting of the p38 pathway, highlighting their relevance in both basic research and drug development.
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