| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NR4A1; GFRP1; HMR; NAK1; Nuclear receptor subfamily 4 group A member 1; Early response protein NAK1; Nuclear hormone receptor NUR/77; Nur77; Orphan nuclear receptor HMR; Orphan nuclear receptor TR3; ST-59; Testicular receptor 3 |
| Entrez GeneID | 3164 |
| WB Predicted band size | Calculated MW: 64 kDa; Observed MW: 64 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rat |
| Immunogen | A synthetic peptide of human NUR77 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3-4条关于p38抗体的参考文献示例(文献信息为模拟生成，仅供参考)：
1. **文献名称**：*Targeting p38 MAPK in inflammatory diseases: A review of inhibitors and antibody-based tools*
**作者**：Cuenda A, Cohen P
**摘要**：综述了p38 MAPK在炎症性疾病中的作用机制，并总结了基于抗体和小分子抑制剂的靶向治疗策略，强调特异性抗体在基础研究和临床诊断中的应用。
2. **文献名称**：*p38α isoform-specific antibodies reveal differential activation in stress responses*
**作者**：Risco A, Cuenda A
**摘要**：研究开发了针对p38α亚型的特异性抗体，验证其在Western blot、免疫沉淀中的有效性，并证明其在氧化应激和细胞凋亡模型中的信号通路检测作用。
3. **文献名称**：*Phospho-p38 antibody as a prognostic marker in cancer progression*
**作者**：Kumar S, et al.
**摘要**：通过免疫组化分析磷酸化p38(Thr180/Tyr182)在肿瘤组织中的表达水平，发现其与患者生存率显著相关，提示p38抗体在癌症预后评估中的潜在价值。
4. **文献名称**：*Generation and characterization of monoclonal antibodies against active p38 MAPK*
**作者**：Cano E, Mahadevan LC
**摘要**：首次报道了针对活性p38 MAPK的单克隆抗体制备，验证其特异性识别磷酸化形式的能力，并用于检测细胞应激(如UV辐射)后的激酶激活状态。
(注：以上文献为示例，实际引用需核实真实存在的论文信息。)
The p38 antibody is a crucial tool for studying the p38 mitogen-activated protein kinase (MAPK) pathway, a signaling cascade involved in cellular responses to stress, inflammation, and apoptosis. Discovered in the 1990s, p38 MAPK belongs to the serine/threonine kinase family and is activated by stressors like cytokines, UV radiation, osmotic shock, or heat. It regulates processes such as cell differentiation, survival, and immune responses by phosphorylating downstream targets, including transcription factors (e.g., ATF-2. CREB) and kinases (e.g., MAPKAPK2).
Antibodies targeting p38 are widely used in research to detect protein expression, phosphorylation status (e.g., p-p38 at Thr180/Tyr182), and localization in tissues or cultured cells. They are essential for techniques like Western blotting, immunofluorescence, and flow cytometry. p38 has four isoforms (α, β, γ, δ) with distinct roles; p38α is the most studied due to its involvement in inflammatory diseases and cancer. Dysregulation of p38 signaling is linked to conditions like rheumatoid arthritis, neurodegenerative disorders, and tumor progression, making these antibodies valuable for mechanistic studies and drug development.
Commercial p38 antibodies are often validated for specificity and sensitivity, though cross-reactivity between isoforms or phosphorylated/non-phosphorylated forms requires careful validation. Their applications extend to evaluating therapeutic inhibitors targeting the p38 pathway in preclinical models.
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