| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | LI; KTG; LI1; TGK; ICR2; ARCI1; TGASE |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human TGM1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是基于典型研究主题模拟的KMT4(PRDM2)抗体相关参考文献示例，供参考(建议通过学术数据库验证最新文献)：
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1. **"A monoclonal antibody targeting KMT4/PRDM2 reveals its role in histone H3 lysine 36 methylation"**
*作者：Zhang L, et al.*
摘要：本研究开发了一种特异性识别KMT4的单克隆抗体，验证了其在Western blot和免疫荧光中的应用，并证明KMT4通过催化H3K36甲基化调控基因沉默。
2. **"PRDM2 antibody-based profiling identifies tumor-suppressive functions in breast cancer"**
*作者：Wang Y, et al.*
摘要：通过免疫组化分析乳腺癌组织，发现KMT4蛋白表达与患者预后相关，抗体验证显示其通过表观遗传机制抑制肿瘤侵袭。
3. **"Characterization of KMT4 knockout mice using allele-specific antibodies"**
*作者：Kimura S, et al.*
摘要：利用KMT4特异性抗体研究基因敲除小鼠模型，揭示其在胚胎发育中调控神经基因甲基化的关键作用。
4. **"Cross-species reactivity analysis of anti-KMT4 antibodies in chromatin studies"**
*作者：Garcia-Beltran C, et al.*
摘要：评估多种KMT4抗体的物种交叉反应性，优化染色质免疫沉淀(ChIP)流程，用于人类和小鼠模型中组蛋白修饰研究。
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**注意**：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索关键词(如“KMT4 antibody”“PRDM2 antibody”)获取最新信息。
The KMT4 antibody targets the KMT4 protein, also known as DOT1L (Disruptor of Telomeric Silencing 1-Like), a conserved histone methyltransferase that specifically catalyzes the methylation of histone H3 on lysine 79 (H3K79). This epigenetic modification plays a critical role in transcriptional regulation, DNA damage repair, and cell cycle progression. Unlike many histone methyltransferases, DOT1L/KMT4 does not contain a SET domain but instead utilizes a unique catalytic mechanism. Its activity is linked to the methylation of H3K79 in euchromatin, influencing gene activation and elongation. Dysregulation of DOT1L/KMT4 is associated with aggressive cancers, particularly mixed-lineage leukemia (MLL)-rearranged leukemias, where aberrant H3K79 methylation drives oncogenic gene expression.
The KMT4 antibody is widely used in research to investigate DOT1L's function through techniques like chromatin immunoprecipitation (ChIP), Western blotting, and immunofluorescence. It helps identify DOT1L's interaction partners, genomic localization, and role in disease pathways. Inhibitors targeting DOT1L/KMT4. such as EPZ-5676. have shown therapeutic potential in clinical trials, underscoring the protein's biomedical relevance. As a tool, the KMT4 antibody aids in unraveling the complex epigenetic networks governing cellular identity and disease, making it vital for studies in oncology, developmental biology, and chromatin dynamics.
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