| WB | 1/500-1/1000 | Mouse,Rat |
| IF | 1/20 | Mouse,Rat |
| IHC | 1/50-1/100 | Mouse,Rat |
| ICC | 技术咨询 | Mouse,Rat |
| FCM | 咨询技术 | Mouse,Rat |
| Elisa | 咨询技术 | Mouse,Rat |
| Aliases | GLUT5, GLUT-5 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human SLC2A5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于TrkB抗体的模拟参考文献，内容基于典型研究场景，供参考：
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1. **文献名称**：*Development and Validation of a High-Specificity TrkB Monoclonal Antibody for Neurodegenerative Research*
**作者**：Chen, L., et al.
**摘要**：本研究报道了一种新型TrkB单克隆抗体的开发，通过免疫印迹(Western blot)和免疫荧光验证了其在啮齿类及人类脑组织中的特异性。该抗体可识别TrkB全长(145 kDa)及截短型(95 kDa)蛋白，适用于检测阿尔茨海默病模型中TrkB表达的下调。
2. **文献名称**：*TrkB Receptor Phosphorylation Dynamics in Hippocampal Neurons Revealed by Phospho-Specific Antibodies*
**作者**：Martinez, A., et al.
**摘要**：作者开发了针对TrkB酪氨酸磷酸化位点(Y515和Y816)的抗体，并利用其探究BDNF刺激下海马神经元TrkB的激活机制。结果显示，Y816磷酸化与神经元突触可塑性密切相关，为TrkB信号通路研究提供了工具。
3. **文献名称**：*Comparative Analysis of Commercial TrkB Antibodies in Post-Mortem Human Brain Tissue*
**作者**：Kim, S., & Reynolds, R.
**摘要**：该研究评估了5种市售TrkB抗体在人类脑组织中的表现，发现仅两种抗体(克隆号：EPR1293和7D8)在免疫组化中具有高特异性，强调抗体选择对抑郁症和癫痫相关TrkB蛋白定位研究的重要性。
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这些文献示例涵盖了抗体开发、信号机制解析及商业化抗体比较等方向，符合TrkB抗体研究的主要应用场景。如需真实文献，建议通过PubMed或Google Scholar检索关键词(如“TrkB antibody validation”)。
Trk B antibody is a crucial tool in neuroscience and cancer research, targeting the tropomyosin receptor kinase B (Trk B), a key receptor tyrosine kinase encoded by the *NTRK2* gene. Trk B primarily binds brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5 (NT-4/5), activating downstream signaling pathways (e.g., MAPK, PI3K/Akt) that regulate neuronal survival, differentiation, synaptic plasticity, and cognitive functions. Dysregulation of Trk B signaling is implicated in neurological disorders (e.g., Alzheimer’s, depression) and cancers, particularly when *NTRK2* fusions drive oncogenic signaling.
Trk B antibodies are used to detect receptor expression, phosphorylation status, and localization in tissues or cells via techniques like Western blot, immunohistochemistry (IHC), and immunofluorescence (IF). In cancer diagnostics, they help identify Trk B overexpression or fusion proteins, guiding targeted therapies (e.g., larotrectinib, entrectinib). Researchers also employ these antibodies to study neurodevelopmental processes and evaluate therapeutic responses in preclinical models.
Available as monoclonal or polyclonal variants, Trk B antibodies vary in specificity and applications. Validation using knockout controls or blocking peptides is critical to ensure reliability. Their role extends to biomarker discovery and mechanistic studies, bridging basic research and clinical translation in neuropathologies and precision oncology.
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