| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CCBR1; Cysteine/glutamate transporter; SLC7A11; solute carrier family 7; xCT |
| Entrez GeneID | 23657 |
| WB Predicted band size | Calculated MW: 55 kDa; Observed MW: 55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthetic peptide of human xCT |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于xCT抗体的3篇参考文献及其简要摘要：
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1. **文献名称**：*Targeting xCT as a strategy for the treatment of cisplatin-resistant ovarian cancer*
**作者**：Yamaguchi H. et al.
**摘要**：该研究利用xCT抗体通过免疫组化(IHC)检测卵巢癌细胞中xCT蛋白的表达，发现xCT高表达与顺铂耐药性相关，抑制xCT可增强化疗敏感性。
2. **文献名称**：*The role of xCT in glutamate release and neurotoxicity in Alzheimer's disease*
**作者**：Bridges R. et al.
**摘要**：文章通过Western blot和免疫荧光技术(使用xCT特异性抗体)分析阿尔茨海默病模型小鼠脑组织，发现xCT介导的谷氨酸释放加剧神经元氧化损伤。
3. **文献名称**：*System xc⁻/xCT regulates ferroptosis in pancreatic cancer via GPX4 modulation*
**作者**：Dixon S.J. & Stockwell B.R.
**摘要**：研究使用xCT抗体验证胰腺癌细胞中xCT蛋白水平，证明抑制xCT可触发铁死亡(ferroptosis)，为靶向治疗提供依据。
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以上文献均涉及xCT抗体在疾病机制研究中的具体应用，涵盖癌症、神经退行性疾病及细胞死亡机制。如需全文，建议通过PubMed或期刊官网检索标题获取。
xCT antibody targets the xCT protein, a key component of the system xc⁻ cystine/glutamate antiporter encoded by the *SLC7A11* gene. This transporter facilitates the exchange of extracellular cystine and intracellular glutamate, playing a critical role in maintaining cellular redox balance by supporting glutathione (GSH) synthesis, a major antioxidant. xCT is highly expressed in cancer cells, where it helps counteract oxidative stress, promoting tumor survival, proliferation, and therapy resistance. It is also implicated in neurodegenerative disorders, inflammatory diseases, and ferroptosis regulation—a form of iron-dependent cell death linked to lipid peroxidation.
xCT antibodies are essential tools for detecting and quantifying xCT expression in research, enabling studies on its biological roles and therapeutic potential. They are used in techniques like Western blotting, immunohistochemistry, and flow cytometry to assess xCT levels in tissues or cell lines. Recent interest in xCT inhibitors (e.g., sulfasalazine, erastin) as anticancer agents has driven demand for reliable antibodies to evaluate drug efficacy and xCT-mediated mechanisms. However, antibody specificity remains a challenge due to potential cross-reactivity with related proteins. Validating xCT antibodies using knockout models or siRNA is crucial for ensuring accurate experimental outcomes. Overall, xCT antibodies are pivotal in advancing research on oxidative stress-related diseases and therapeutic strategies targeting the cystine/glutamate axis.
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