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Rabbit Monoclonal SQSTM1/p62 Antibody

  • 中文名： SQSTM1/p62抗体
  • 别    名： p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3
货号: IPDX22728
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 1/20 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

Aliasesp60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3
Entrez GeneID8878
WB Predicted band sizeCalculated MW: 48 kDa; Observed MW: 62 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse
ImmunogenRecombinant protein of human SQSTM1
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是关于SQSTM1/p62抗体的3篇参考文献，按格式整理：

1. **文献名称**：*"p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy"*

**作者**：Ichimura Y, Kumanomidou T, Sou YS, et al.

**摘要**：该研究通过免疫共沉淀和荧光标记技术，揭示了p62通过其LC3相互作用区(LIR)直接结合自噬关键蛋白LC3.促进泛素化蛋白聚集体的自噬降解。实验中采用p62抗体进行Western blot和免疫荧光验证。

2. **文献名称**：*"Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice"*

**作者**：Komatsu M, Kageyama S, Ichimura Y.

**摘要**：研究利用p62基因敲除小鼠模型，通过免疫组化和抗体染色发现，p62缺失可显著减少自噬缺陷小鼠肝脏中的蛋白聚集体，证明p62在调控细胞质内含体形成中的关键作用。p62抗体用于组织切片染色和定量分析。

3. **文献名称**：*"Sequestosome 1/p62 is a polyubiquitin chain binding protein involved in ubiquitin proteasome degradation"*

**作者**：Bjørkøy G, Lamark T, Brech A, et al.

**摘要**：该研究通过免疫印迹和免疫电镜技术，首次证实p62作为泛素链结合蛋白参与蛋白酶体降解途径。实验中利用p62抗体追踪其在泛素化蛋白聚集区域的定位，并验证其与泛素化蛋白的相互作用。

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**注**：以上文献均聚焦于p62在蛋白降解途径中的作用机制，抗体应用涵盖Western blot、免疫荧光及组织染色等实验场景。如需具体期刊信息或发表日期，可进一步补充检索。

背景信息

The SQSTM1/p62 antibody is a crucial tool for studying the multifunctional adaptor protein p62 (sequestosome 1), encoded by the SQSTM1 gene. p62 serves as a scaffolding protein involved in diverse cellular processes, including autophagy, apoptosis, and signal transduction pathways such as NF-κB and NRF2. It interacts with ubiquitinated proteins, directing them to autophagosomes for degradation, making it a key marker for monitoring autophagic flux. Dysregulation of p62 is linked to various diseases, including cancer, neurodegenerative disorders (e.g., Alzheimer’s, Parkinson’s), and metabolic conditions. In autophagy-deficient cells, p62 accumulates due to impaired degradation, which can be detected using p62-specific antibodies. Researchers employ SQSTM1/p62 antibodies in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression, localization, and aggregation in pathological contexts. These antibodies often target specific domains, such as the ubiquitin-associated (UBA) region or PB1 domain, enabling functional studies of p62’s role in protein turnover and stress responses. Its association with inclusion bodies in neurodegenerative diseases and upregulation in certain cancers underscores its diagnostic and therapeutic relevance. Validation of antibody specificity is critical, as p62 interacts with numerous binding partners and post-translational modifications may influence detection. Overall, SQSTM1/p62 antibodies are indispensable for exploring cellular quality control mechanisms and disease pathogenesis.

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