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Rabbit Monoclonal RIP Antibody

  • 中文名: RIP抗体
  • 别    名: RIPK1; Cell death protein RIP; RIP1; RIP; RIP-1; Rinp
货号: IPDX22726
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesRIPK1; Cell death protein RIP; RIP1; RIP; RIP-1; Rinp
Entrez GeneID8737
WB Predicted band sizeCalculated MW: 76 kDa; Observed MW: 76 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenRecombinant protein of human RIP
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是关于RIP抗体的3篇代表性文献及其摘要概括:

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1. **文献名称**:*Identification of RIP1 kinase as a specific cellular target of necroptosis*

**作者**:Degterev, A. et al.

**摘要**:该研究通过开发特异性RIP1(Receptor-interacting protein 1)抗体,揭示了其在坏死性凋亡(necroptosis)信号通路中的关键作用。实验表明,RIP1与坏死复合体(necrosome)的形成直接相关,并验证了该抗体在检测内源性RIP1激活状态中的应用。

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2. **文献名称**:*RIP3. an energy metabolism regulator that switches TNF-induced cell death from apoptosis to necrosis*

**作者**:He, S. et al.

**摘要**:本文利用RIP3特异性抗体,阐明了RIP3通过调控能量代谢决定细胞死亡方式(凋亡或坏死)的机制。研究发现,RIP3缺失的细胞无法形成坏死复合体,且抗体检测证实其与RIP1的相互作用依赖于特定磷酸化事件。

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3. **文献名称**:*Functional complementation between FADD and RIP1 in embryogenesis and NF-κB signaling*

**作者**:Kelliher, M.A. et al.

**摘要**:通过RIP1基因敲除模型及相应抗体的应用,研究揭示了RIP1在胚胎发育和NF-κB信号通路中的双重功能。免疫共沉淀实验表明,RIP1抗体可特异性识别其与死亡结构域蛋白(如TRADD)的结合,为RIP1在炎症反应中的作用提供证据。

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4. **文献名称**:*RIP1 suppresses innate immune necrotic as well as apoptotic cell death during mammalian development*

**作者**:Dillon, C.P. et al.

**摘要**:该研究利用条件性RIP1敲除小鼠及特异性抗体,证明RIP1通过抑制坏死和凋亡双重途径维持组织稳态。Western blot和免疫组化结果显示,RIP1缺失导致自发性的细胞死亡,提示其在先天免疫中的保护作用。

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这些文献涵盖了RIP抗体在机制研究、信号通路解析及疾病模型中的应用,涉及凋亡、坏死性凋亡和免疫调控等多个方向。如需具体文章链接或补充更多文献,可进一步说明需求。

背景信息

The Receptor-Interacting Protein (RIP) antibodies are essential tools for studying the RIP kinase family, a group of serine/threonine kinases critical in regulating cell death and inflammation. RIP kinases, particularly RIP1 (RIPK1) and RIP3 (RIPK3), play pivotal roles in necroptosis—a programmed form of necrosis—and modulate apoptosis and NF-κB signaling pathways. RIP1 acts as a key decision-maker in cell survival and death, while RIP3 is indispensable for necroptosis execution through phosphorylation of MLKL. Antibodies targeting RIP kinases enable researchers to detect protein expression, post-translational modifications (e.g., phosphorylation), and subcellular localization via techniques like Western blot, immunohistochemistry, and immunofluorescence. These reagents are vital in exploring diseases linked to dysregulated cell death, such as cancer, neurodegenerative disorders, and inflammatory conditions. Specificity is crucial, as RIP isoforms share structural homology; thus, antibodies are often validated for selective recognition of target epitopes. Additionally, RIP antibodies aid in evaluating therapeutic agents, including RIP kinase inhibitors under investigation for treating inflammatory and degenerative diseases. Their application continues to advance understanding of cellular stress responses and immune regulation.

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