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Rabbit Monoclonal Rad51 Antibody

  • 中文名: Rad51抗体
  • 别    名: RAD51; RAD51A; RECA; DNA repair protein RAD51 homolog 1; HsRAD51; hRAD51; RAD51 homolog A
货号: IPDX22724
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 1/20 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesRAD51; RAD51A; RECA; DNA repair protein RAD51 homolog 1; HsRAD51; hRAD51; RAD51 homolog A
Entrez GeneID5888
WB Predicted band sizeCalculated MW: 37 kDa; Observed MW: 37 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthetic peptide of human Rad51
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是关于Rad51抗体的3篇参考文献摘要,按研究重点分类整理:

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### 1. **抗体开发与验证**

**文献名称**:*Production and characterization of monoclonal antibodies specific for the human Rad51 protein*

**作者**:Westerman, L.S. et al.

**摘要**:本研究报道了针对人源Rad51蛋白的单克隆抗体的开发。通过免疫印迹(Western blot)和免疫荧光验证,证明该抗体能特异性识别重组及内源性Rad51.适用于检测DNA损伤后细胞核内Rad51焦点的形成。

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### 2. **DNA修复机制研究**

**文献名称**:*Association of BRCA1 with Rad51 in mitotic and meiotic cells*

**作者**:Scully, R. et al.

**摘要**:利用Rad51抗体进行共聚焦显微镜分析,揭示BRCA1与Rad51在DNA损伤修复过程中的共定位。研究指出,BRCA1缺陷细胞中Rad51焦点形成受损,提示两者在维持基因组稳定性中的协同作用。

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### 3. **抗体的功能应用**

**文献名称**:*Rad51-deficient vertebrate cells accumulate chromosomal breaks prior to cell death*

**作者**:Pierce, A.J. et al.

**摘要**:通过Rad51抗体进行免疫荧光染色,证实Rad51缺失的脊椎动物细胞在DNA双链断裂后无法有效修复损伤,导致染色体异常累积。研究强调了Rad51在哺乳动物细胞同源重组修复中的必要性。

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### 可选补充(机制探索)

**文献名称**:*BRCA1 phosphorylation by Aurora-A is essential for prophase I arrest in mammalian oocytes*

**作者**:T. Taniguchi et al.

**摘要**:采用Rad51抗体分析卵母细胞减数分裂进程,发现BRCA1磷酸化缺陷导致Rad51焦点组装异常,阻碍同源重组修复,最终引发染色体分离错误。

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**提示**:建议优先阅读Westerman (1997)了解抗体特性,再结合Scully或Pierce的研究深入DNA修复机制。如需实验方案,可进一步检索抗体货号及具体应用条件。

背景信息

The Rad51 antibody is a crucial tool in studying homologous recombination (HR), a vital DNA repair pathway maintaining genomic stability. Rad51. a eukaryotic homolog of bacterial RecA, plays a central role in HR by catalyzing strand pairing and exchange between damaged DNA and intact homologous templates. It forms nucleoprotein filaments on single-stranded DNA (ssDNA), facilitating homology search and strand invasion. Dysregulation of Rad51 is linked to cancer predisposition, therapy resistance, and developmental disorders. Rad51 antibodies are widely used to detect Rad51 foci formation (a marker of HR activity) via immunofluorescence, quantify protein levels by Western blot, or assess spatial-temporal dynamics in cellular responses to DNA damage. These applications help evaluate HR efficiency, study chemotherapeutic responses (e.g., PARP inhibitor sensitivity in BRCA-deficient cancers), and explore replication stress mechanisms. Commercially available Rad51 antibodies vary in specificity across species (human, mouse, etc.) and applications, with some clones recognizing conserved epitopes. Validation is critical, as nonspecific cross-reactivity with other RecA-family proteins (e.g., Rad51 paralogs) may occur. Recent studies also utilize Rad51 antibodies in spatial transcriptomics and super-resolution microscopy to dissect DNA repair compartmentalization. Its role in cancer biology and therapy optimization makes Rad51 antibody a cornerstone reagent in molecular oncology and DNA repair research.

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