首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人鸢尾素酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的irisin含量。Irisin是一种由运动诱导产生的肌肉因子。背景上，它被视为运动益处的重要介质。研究机制方面，Irisin能促进白色脂肪棕色化，增加能量消耗，还参与调节葡萄糖代谢、骨代谢等过程，在代谢性疾病等领域有潜在治疗价值。试剂盒检测范围为3.12 ng/mL-200 ng/mL，科研人员可将其应用于运动生理学、代谢调控机制、脂肪组织功能研究等领域，通过定量分析不同干预条件下（如运动训练、药物处理）样本中鸢尾素浓度变化，为探索代谢相关疾病的分子机制提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：irisin
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human irisin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||||||||
| Sample | Serum(n=4) | ||||||||||||
| 1:1 | Average % | 90 | |||||||||||
| Range % | 84-96 | ||||||||||||
| 1:2 | Average % | 97 | |||||||||||
| Range % | 91-103 | ||||||||||||
| 1:4 | Average % | 103 | |||||||||||
| Range % | 97-109 | ||||||||||||
| 1:8 | Average % | 89 | |||||||||||
| Range % | 83-95 | ||||||||||||
回收率：
| The recovery of human irisin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||||||||
| Sample Type | Average % Recovery | Range | |||||||||||
| Serum (n=5) | 100 | 94-106 | |||||||||||
| EDTA plasma (n=4) | 106 | 101-112 | |||||||||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||
| ng/mlOD1OD2AverageCorrected 2002.6902.7522.7212.616 1002.4022.3712.3872.282 501.9651.8531.9091.804 251.3551.2471.3011.196 12.50.7640.7350.7500.645 6.250.4950.4640.4800.375 3.120.2510.2660.2590.154 00.1060.1030.105 | |||||||||||||
货期：3-5 working days
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