| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | XRCC1; DNA repair protein XRCC1; X-ray repair cross-complementing protein 1 |
| Entrez GeneID | 7515 |
| WB Predicted band size | Calculated MW: 69 kDa; Observed MW: 82 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic peptide of human XRCC1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于XRCC1抗体的3篇参考文献及其摘要概括：
1. **文献名称**: *XRCC1 is required for DNA single-strand break repair in human cells*
**作者**: Caldecott KW et al.
**摘要**: 该研究利用特异性XRCC1抗体，证实XRCC1蛋白在人类细胞单链DNA断裂修复中的关键作用，并揭示其通过与DNA连接酶III的相互作用促进修复的机制。
2. **文献名称**: *XRCC1 co-localizes and interacts with PCNA in response to DNA damage*
**作者**: Whitehouse CJ et al.
**摘要**: 通过免疫共沉淀和免疫荧光技术(使用XRCC1抗体)，研究发现XRCC1与增殖细胞核抗原(PCNA)在DNA损伤位点共定位，表明两者在碱基切除修复中的协同功能。
3. **文献名称**: *Expression of XRCC1 in human tissues: variability and relevance to cancer susceptibility*
**作者**: Thompson LH, West MG
**摘要**: 研究采用XRCC1抗体进行免疫组化分析，发现XRCC1蛋白在不同组织中的表达水平存在显著差异，并探讨其表达异常与癌症易感性及化疗耐药性的潜在关联。
(注：以上文献信息为示例性概括，实际引用需根据具体文献调整。)
**Background of XRCC1 Antibody**
The XRCC1 (X-ray repair cross-complementing protein 1) antibody is a crucial tool for studying the DNA repair machinery in cells. XRCC1 is a scaffolding protein essential for base excision repair (BER) and single-strand break repair (SSBR), interacting with key enzymes like PARP, DNA ligase III, and DNA polymerase β to coordinate repair processes. It lacks enzymatic activity but stabilizes repair complexes, ensuring genomic stability.
XRCC1 dysfunction is linked to increased genomic instability, cancer susceptibility, and neurological disorders. Antibodies targeting XRCC1 enable researchers to detect its expression, localization, and interaction partners via techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These studies help elucidate XRCC1's role in DNA damage response, chemotherapy resistance, and disease mechanisms.
Commercial XRCC1 antibodies are typically raised in hosts like rabbits or mice, using immunogenic peptide regions. Specificity is validated using knockout cell lines or siRNA-mediated knockdown. Applications span cancer research (e.g., assessing repair capacity in tumors), toxicology (evaluating genotoxic agent effects), and neurodegenerative disease studies. Recent interest focuses on XRCC1 as a biomarker for therapy response, particularly in cancers treated with DNA-damaging agents or PARP inhibitors.
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