| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CAHL; hYTHDC2 |
| Entrez GeneID | 240255 |
| WB Predicted band size | Calculated MW: 161 kDa; Observed MW: 161 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of mouse YTHDC2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于YTHDC2抗体的3篇参考文献示例(注：文献为虚拟示例，实际引用需核实)：
1. **文献名称**: *YTHDC2 regulates germline mRNA stability and translation during spermatogenesis*
**作者**: Hsu, P.J., et al.
**摘要**: 该研究利用YTHDC2特异性抗体，通过免疫沉淀和Western blot技术，揭示了YTHDC2通过结合mRNA的m⁶A修饰调控小鼠精子发生的关键作用，其缺失导致雄性不育。
2. **文献名称**: *YTHDC2 promotes tumor progression in hepatocellular carcinoma via EGFR signaling*
**作者**: Wang, L., et al.
**摘要**: 研究通过YTHDC2抗体进行免疫组化分析，发现YTHDC2在肝癌组织中高表达，且与患者预后不良相关，其通过调控EGFR通路促进肿瘤侵袭。
3. **文献名称**: *Structural basis of YTHDC2-RNA recognition and its role in translational repression*
**作者**: Bailey, A.S., et al.
**摘要**: 结合YTHDC2抗体的免疫荧光实验，阐明了YTHDC2蛋白的RNA结合结构域及其在细胞质中抑制靶标mRNA翻译的分子机制。
(提示：实际文献需通过PubMed/Google Scholar等平台以“YTHDC2 antibody”为关键词检索，并筛选涉及抗体应用的实验研究。)
YTHDC2 (YTH domain-containing protein 2) is an RNA-binding protein belonging to the YTH family, which recognizes N6-methyladenosine (m6A), a prevalent post-transcriptional modification in eukaryotic RNA. YTHDC2 is distinct from other YTH proteins due to its unique structural features, including an N-terminal DEAD-box helicase domain and a C-terminal YTH domain, enabling both RNA unwinding and m6A-dependent RNA regulation. It plays critical roles in RNA metabolism, including translation enhancement, mRNA stability modulation, and involvement in meiotic processes. Studies highlight its importance in germ cell development, particularly spermatogenesis, where it regulates the expression of key meiotic genes. Dysregulation of YTHDC2 has been linked to infertility and cancer progression, with its overexpression observed in malignancies like hepatocellular carcinoma.
YTHDC2 antibodies are essential tools for investigating these biological and pathological mechanisms. They enable detection of YTHDC2 expression and localization in tissues or cultured cells through techniques like Western blotting, immunohistochemistry, and immunofluorescence. Specific antibodies also facilitate studies on YTHDC2's interaction partners, post-translational modifications, and its role in m6A-mediated pathways. Commercially available antibodies are typically raised against epitopes in the N-terminal or YTH domain regions, with validation including knockout controls to ensure specificity. Ongoing research using these antibodies continues to unravel YTHDC2's dual functions as an m6A reader and RNA helicase, bridging epitranscriptomic regulation with cellular energy dynamics and disease pathogenesis.
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