| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | hVPS35; MEM3; PARK17; VPS35 |
| Entrez GeneID | 55737 |
| WB Predicted band size | Calculated MW: 92 kDa; Observed MW: 81 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human VPS35 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于VPS35抗体的3篇参考文献，涵盖其在不同疾病模型中的应用及机制研究：
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1. **文献名称**：*VPS35 mutations in Parkinson disease*
**作者**：Vilariño-Güell C, et al. (2011)
**摘要**：该研究首次报道了VPS35基因D620N突变与家族性帕金森病的关联。通过免疫印迹和免疫组化实验，利用VPS35抗体证实突变导致蛋白质表达异常，并影响逆转录复合体功能，进而参与神经元变性机制。
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2. **文献名称**：*VPS35 regulates APP trafficking and processing in Alzheimer's disease*
**作者**：Wen L, et al. (2011)
**摘要**：研究探讨VPS35在阿尔茨海默病中对淀粉样前体蛋白(APP)运输的影响。通过VPS35抗体进行免疫沉淀和免疫荧光实验，发现其缺失导致APP异常分选，促进β-淀粉样蛋白生成，提示VPS35在神经退行中的保护作用。
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3. **文献名称**：*VPS35 dysfunction impairs lysosomal degradation and promotes α-synuclein aggregation*
**作者**：Follett J, et al. (2014)
**摘要**：利用VPS35抗体在小鼠模型中研究其在溶酶体途径中的作用，发现VPS35缺失导致α-突触核蛋白清除障碍，加剧神经元内包涵体形成，为帕金森病病理机制提供新见解。
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4. **文献名称**：*Structural basis for antibody recognition in the VPS35-retromer assembly*
**作者**：Zavodszky E, et al. (2014)
**摘要**：通过冷冻电镜和抗体结合实验解析VPS35-retromer复合体的结构，揭示抗体识别VPS35的关键表位，为开发靶向VPS35的分子工具及疾病治疗策略提供结构基础。
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以上文献展示了VPS35抗体在疾病机制、蛋白质功能及结构研究中的关键作用。如需具体文章信息，建议通过PubMed或期刊数据库查询完整文本。
VPS35 (Vacuolar Protein Sorting 35 homolog) is a critical component of the retromer complex, a conserved protein assembly involved in intracellular trafficking and endosome-to-Golgi retrieval of membrane-associated proteins. It plays a key role in sorting cargo proteins, such as lysosomal enzymes and signaling receptors, ensuring proper cellular function. Dysregulation of VPS35 is linked to neurodegenerative diseases, notably Parkinson’s disease, where specific mutations (e.g., D620N) are associated with autosomal dominant familial forms. It also intersects with pathways implicated in Alzheimer’s disease, cancer metastasis, and viral infection mechanisms.
VPS35 antibodies are essential tools for studying its expression, localization, and interaction partners. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate tissue-specific distribution, protein levels in disease models, or retromer complex dynamics. Researchers employ these antibodies to explore VPS35’s role in neuronal survival, synaptic function, and autophagy. Commercial VPS35 antibodies are typically raised against specific epitopes (human or mouse variants) and validated for cross-reactivity across species. Challenges include ensuring specificity due to homology with other sorting proteins and distinguishing between wild-type and mutant forms in pathological studies. Recent studies also leverage CRISPR/Cas9-engineered models to correlate VPS35 antibody findings with functional genomics data.
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