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产品描述：猪N端前脑钠素(NT-proBNP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NT-proBNP含量。NT - proBNP即N末端B型利钠肽原，是心室肌细胞分泌的BNP前体裂解产物，主要用于诊断心力衰竭和判断预后。，心衰时心室壁张力增加，刺激NT - proBNP释放，通过检测其血浆水平变化为病情评估提供依据。试剂盒检测范围为7.8 pg/mL-500 pg/mL，猪心血管疾病模型研究、心脏病理机制探索、药物干预效果评估、实验室开展猪心脏疾病模型构建、心肌病机制研究、心血管药物筛选、深入解析心脏疾病的分子调控机制本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：NT-proBNP
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig NT-proBNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 103 | ||||
| Range % | 96-108 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 91-102 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 86-98 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 90-102 | |||||
回收率：
| The recovery of pig NT-proBNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 105 | 99-109 | ||||
| EDTA plasma (n=4) | 97 | 90-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.1292.0452.0871.9492501.6711.5551.6131.4751251.1341.1171.1260.98862.50.6660.6500.6580.52031.20.3880.3710.3800.24215.60.2570.2660.2620.1247.80.1710.1670.1690.03100.1370.1390.138 | ||||||
货期：3-5 working days
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