| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KAPG; PKACg |
| Entrez GeneID | 5568 |
| clone | 2E4 |
| WB Predicted band size | 40.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PRKACG expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TPX2抗体的3篇参考文献的简要信息：
1. **文献名称**：*"TPX2: A key regulator of spindle assembly and cancer progression"*
**作者**：Schultz, S.J. et al.
**摘要**：研究阐明了TPX2在有丝分裂中调控纺锤体组装的功能，并利用特异性抗体通过免疫荧光和Western blot验证其在多种癌细胞系中的高表达，提示其与肿瘤发生相关。
2. **文献名称**：*"Localization of TPX2 during mitosis and its role in Aurora A kinase activation"*
**作者**：Gruss, O.J. et al.
**摘要**：通过免疫组化和TPX2抗体标记，发现TPX2在核膜破裂后定位于纺锤体极体，并证明其通过激活Aurora A激酶调控染色体分离，为靶向治疗提供依据。
3. **文献名称**：*"Overexpression of TPX2 correlates with poor prognosis in hepatocellular carcinoma"*
**作者**：Neumayer, G. et al.
**摘要**：利用TPX2抗体对肝癌组织样本进行免疫组化分析，发现TPX2的高表达与患者生存率降低显著相关，提示其作为肝癌预后标志物的潜力。
4. **文献名称**：*"Functional analysis of TPX2 in microtubule nucleation using RNAi and antibody-based approaches"*
**作者**：Karlsson, C. et al.
**摘要**：通过抗体阻断和RNA干扰技术，证实TPX2在微管成核中的必要性，并揭示其在维持基因组稳定性中的作用。
(注：以上文献为模拟概括，实际引用时需核实具体文献信息。)
The TPX2 (Targeting Protein for Xklp2) antibody is a crucial tool in studying the biological functions of the TPX2 protein, a key regulator of mitotic spindle assembly and cell cycle progression. First identified in Xenopus laevis, TPX2 is conserved across eukaryotes and plays a dual role in orchestrating microtubule nucleation during mitosis and activating Aurora A kinase, a critical driver of mitotic entry. Its dysregulation is strongly linked to genomic instability and cancer progression, with overexpression observed in various malignancies, including breast, liver, and pancreatic cancers.
TPX2 antibodies are primarily used in research to detect and quantify TPX2 expression in cells or tissues, enabling investigations into its spatial-temporal dynamics during mitosis. These antibodies facilitate techniques like immunofluorescence, Western blotting, and immunohistochemistry, helping visualize TPX2's localization to spindle poles and microtubules. In clinical contexts, TPX2 antibodies have potential diagnostic value, as elevated TPX2 levels correlate with tumor aggressiveness and poor prognosis. Recent studies also explore TPX2 as a therapeutic target, with antibodies aiding in validating knockdown strategies or inhibitor efficacy.
Challenges remain in ensuring antibody specificity due to TPX2's post-translational modifications and structural homology with other mitotic proteins. Nonetheless, TPX2 antibodies remain indispensable for unraveling its roles in cell division and cancer biology.
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