首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠低密度脂蛋白胆固醇(LDL-C)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LDL-C含量。LDL-C（低密度脂蛋白胆固醇）是心血管疾病重要风险因素。其水平升高易在血管壁沉积形成斑块。科研聚焦降低LDL-C水平以减少心血管事件。研究机制包括抑制合成、加速清除等，如开发他汀类、PCSK9抑制剂等药物来干预该靶点。试剂盒检测范围为0.312 μg/mL-20 μg/mL，该产品可广泛应用于小鼠模型中的脂质代谢机制研究、心血管疾病相关药物开发、饮食干预实验或基因工程动物模型的表型分析，为评估脂质代谢异常、动脉硬化病理进程及潜在治疗策略提供可靠的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：LDL-C
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 μg/mL-20 μg/mL
灵敏度：0.078 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse LDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 86 | |||||
| Range % | 82-92 | ||||||
| 1:200 | Average % | 94 | |||||
| Range % | 91-98 | ||||||
| 1:400 | Average % | 107 | |||||
| Range % | 103-114 | ||||||
| 1:800 | Average % | 89 | |||||
| Range % | 85-94 | ||||||
回收率：
| The recovery of mouse LDL-Cspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 88 | 84-93 | |||||
| EDTA plasma (n=4) | 91 | 87-95 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| μg/mlOD1OD2Average 200.1050.1070.106 100.1730.1780.176 50.2480.2550.252 2.50.3300.3450.338 1.250.4300.4430.437 0.6250.6400.6820.661 0.3120.9411.0110.976 01.7521.8341.793 | |||||||
货期：3-5 working days
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