首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠胰α淀粉酶(AMY2A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的AMY2A含量。AMY2A是一种与淀粉酶相关的靶点。它在人体消化过程中发挥作用，能将淀粉分解为糖类。研究机制上，聚焦于其基因表达调控、酶活性影响因素等，探索其与消化系统疾病的关联，有望为相关疾病的诊断和治疗提供新方向。试剂盒检测范围为9.38 mU/mL-600 mU/mL，适用于体外培养细胞模型、动物实验样本的酶活性评估，以及代谢干预或病理状态下AMY2A动态变化的机制研究，为探索胰腺功能调控及消化系统相关疾病提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Amy2 ELISA Kit; Amy2a ELISA Kit; Pancreatic alpha-amylase ELISA Kit; PA ELISA Kit; EC 3.2.1.1 ELISA Kit; 1,4-alpha-D-glucan glucanohydrolase ELISA Kit
缩写：AMY2A
Uniprot No.：P00688
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：9.38 mU/mL-600 mU/mL
灵敏度：2.3 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse AMY2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 97 | |||||
| Range % | 92-105 | ||||||
| 1:2 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
| 1:4 | Average % | 86 | |||||
| Range % | 80-92 | ||||||
| 1:8 | Average % | 89 | |||||
| Range % | 84-97 | ||||||
回收率：
| The recovery of mouse AMY2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 87-97 | |||||
| EDTA plasma (n=4) | 101 | 94-107 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| mU/mlOD1OD2AverageCorrected6002.6802.7142.6972.5633002.1642.2352.2002.0661501.4671.4211.4441.310750.7590.8110.7850.65137.50.5330.5640.5490.41518.750.3170.3260.3220.1889.380.2270.2410.2340.10000.1280.1390.134 | |||||||
货期：3-5 working days
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