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产品描述：猪补体片断5a(C5a)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C5a含量。C5a是重要的免疫相关靶点。在补体系统激活过程中被裂解产生，具有趋化炎症细胞、调节免疫反应等作用。其异常激活与多种炎症性疾病及自身免疫病相关，目前科研聚焦于研究其信号通路机制，以开发针对性治疗药物。试剂盒检测范围为9.375 ng/mL-600 ng/mL，适用于科研领域对猪源样本中C5a水平的动态监测，例如免疫相关疾病模型研究、感染或炎症状态下补体系统激活程度的评估，以及药物或疫苗开发中免疫调节效应的体外检测，为探索补体介导的病理生理机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：C5a
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：9.375 ng/mL-600 ng/mL
灵敏度：2.34 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 99 | ||||
| Range % | 91-109 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 92-104 | |||||
回收率：
| The recovery of pig C5a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 96 | 92-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected6002.3852.2842.3352.1603001.9031.8021.8531.6781501.3761.3551.3661.191750.9030.9140.9090.73437.50.6500.6610.6560.48118.750.4110.4220.4170.2429.3750.3170.3120.3150.14000.1750.1740.175 | ||||||
货期：3-5 working days
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