WB | 1/500-1/1000 | Human,Mouse,Rat |
IF | 1/20 | Human,Mouse,Rat |
IHC | 1/50-1/100 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | NSP; SARA; MADHIP; SMADIP; PPP1R173 |
Entrez GeneID | 9372 |
WB Predicted band size | Calculated MW: 156 kDa; Observed MW: 156 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | A synthetic peptide of human SARA |
Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于SARA(Smad Anchor for Receptor Activation)抗体的参考文献示例(注:以下内容为示例,非真实文献):
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1. **文献名称**:*SARA regulates TGF-β signaling by recruiting Smads to the activated receptor complex*
**作者**:Tsukazaki T, et al.
**摘要**:研究揭示了SARA蛋白在TGF-β信号通路中的作用,通过其FYVE结构域结合内体膜,介导Smad2/3与激活的TGF-β受体结合,促进信号转导。文中使用SARA抗体验证了其在受体复合体中的定位。
2. **文献名称**:*The role of SARA in fibrosis: Insights from antibody-based functional studies*
**作者**:Xi Q, et al.
**摘要**:通过特异性SARA抗体阻断实验,发现SARA在肝纤维化中调控TGF-β诱导的胶原沉积,表明其作为潜在治疗靶点的价值。
3. **文献名称**:*SARA-dependent endosomal trafficking in cancer metastasis*
**作者**:Di Guglielmo GM, Wrana JL
**摘要**:探讨SARA通过内体运输调控TGF-β信号的空间特异性,并利用SARA抗体证明其在乳腺癌细胞迁移中的关键作用。
4. **文献名称**:*Antibody profiling of SARA isoforms in developmental biology*
**作者**:Liu F, et al.
**摘要**:开发了针对不同SARA异构体的特异性抗体,发现其在胚胎发育过程中时空表达差异,提示其功能多样性。
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**注**:以上文献为示例,实际引用时需根据具体研究领域核实真实文献。如需真实文献,建议通过PubMed或Google Scholar搜索关键词“SARA antibody”或“Smad anchor for receptor activation”。
SARA (Smad anchor for receptor activation) is a cytosolic scaffold protein critical for transforming growth factor-beta (TGF-β) signaling. It facilitates the activation of receptor-regulated Smads (R-Smads) by anchoring them to TGF-β receptor complexes. SARA contains a FYVE domain that directs its localization to early endosomes, where it recruits Smad2/3 to activated TGF-β receptors, enabling their phosphorylation and subsequent signaling. This process regulates diverse cellular responses, including proliferation, differentiation, and apoptosis.
SARA-specific antibodies are essential tools for studying TGF-β pathway dynamics. They enable detection of SARA expression levels, subcellular distribution (particularly endosomal localization), and interactions with Smads or receptors in techniques like Western blotting, immunofluorescence, and co-immunoprecipitation. Research using these antibodies has revealed SARA's role in development and diseases; reduced SARA expression correlates with cancer progression and fibrotic disorders, likely due to impaired Smad activation. Antibodies targeting specific domains (e.g., FYVE or Smad-binding regions) further dissect SARA's functional mechanisms. However, interpretation requires caution due to potential cross-reactivity with homologous proteins. Overall, SARA antibodies remain vital for investigating TGF-β signaling aberrations in pathophysiology and therapeutic targeting.
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