首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人弹性蛋白酶2，中性粒细胞(ELA2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ELA2含量。人弹性蛋白酶2（ELA2）是一种丝氨酸蛋白酶，主要由中性粒细胞分泌，参与炎症反应和宿主防御。其研究机制涉及在白细胞弹性蛋白酶（NE）合成的过程中，异常酶可能导致中性粒细胞破坏，与先天性或周期性中性粒细胞减少症等血液系统疾病相关。试剂盒检测范围为6.25 ng/mL-400 ng/mL，适用于基础科研领域，如炎症机制研究、肺部疾病模型分析或感染性疾病中蛋白表达动态监测，也可用于药物开发过程中靶点蛋白的体外功效评估。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Bone marrow serine protease ELISA Kit; ELA2 ELISA Kit; ELANE ELISA Kit; Elastase 2 ELISA Kit; Elastase 2 neutrophil ELISA Kit; Elastase neutrophil expressed ELISA Kit; Elastase-2 ELISA Kit; ELNE_HUMAN ELISA Kit; GE ELISA Kit; Granulocyte derived elastase ELISA Kit; HLE ELISA Kit; HNE ELISA Kit; Human leukocyte elastase ELISA Kit; Leukocyte elastase ELISA Kit; Medullasin ELISA Kit; NE ELISA Kit; Neutrophil elastase ELISA Kit; PMN E ELISA Kit; PMN elastase ELISA Kit; Polymorphonuclear elastase ELISA Kit; SCN1 ELISA Kit
缩写：ELA2
Uniprot No.：P08246
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ELA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-95 | |||||
| 1:2 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-104 | |||||
回收率：
| The recovery of human ELA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 108 | 98-110 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected4002.4122.3112.3622.2052001.8361.7351.7861.6291001.2131.2021.2081.051500.7040.6930.6990.542250.3640.3540.3590.20212.50.2760.2870.2820.1256.250.2240.2210.2230.06600.1560.1570.157 | ||||||
货期：3-5 working days
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