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产品描述：仓鼠γ干扰素(IFNG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFNG含量。IFNG 即干扰素 -γ，是一种具有多种生物学功能的细胞因子。它在免疫调节、抗病毒和抗肿瘤等方面发挥关键作用。其研究机制主要围绕调节巨噬细胞、T 细胞等免疫细胞的功能，激活免疫信号通路，增强机体的免疫防御能力来展开。试剂盒检测范围为1.87 pg/mL-120 pg/mL，适用于仓鼠感染性疾病模型中的免疫机制研究、疫苗免疫原性评估、肿瘤微环境分析等科研场景，为实验室开展细胞免疫调控研究或炎症反应监测提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IFNGInterferon gamma ELISA Kit; IFN-gamma ELISA Kit
缩写：IFNG
Uniprot No.：O35497
种属：Mesocricetus auratus (Golden hamster)
样本类型：serum, plasma, tissue homogenate
检测范围：1.87 pg/mL-120 pg/mL
灵敏度：0.46 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of hamster IFNG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-94 | |||||
| 1:2 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 94-102 | |||||
回收率：
| The recovery of hamster IFNG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-100 | ||||
| EDTA plasma (n=4) | 92 | 88-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 1202.5842.7272.6562.512 602.0282.1062.0671.923 301.2651.3341.3001.156 150.7140.7550.7350.591 7.50.3710.3470.3590.215 3.750.2930.3090.3010.157 1.870.2070.2170.2120.068 00.1460.1420.144 | ||||||
货期：3-5 working days
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