首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔白介素12(IL-12/P70)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL12/P70含量。IL12/P70是重要的免疫靶点。它由p35和p40亚基组成，在调节免疫细胞功能、促进Th1细胞分化等方面发挥关键作用。其研究机制主要聚焦于调控免疫应答相关信号通路。深入研究它有望为免疫相关疾病治疗提供新策略。试剂盒检测范围为3.12 pg/mL-200 pg/mL，该产品适用于实验动物模型的免疫调控研究、感染性疾病机制探索、疫苗效力评估及炎症相关科研项目，为研究IL-12/P70在免疫微环境中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL12/P70, Interleukin-12/P70
缩写：IL-12/P70
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 89-95 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 83-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 92-102 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-99 | |||||
回收率：
| The recovery of rabbit IL-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 86-110 | ||||
| EDTA plasma (n=4) | 97 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.0962.1082.1021.9381001.6781.6511.6651.501501.2541.2371.2461.082250.8960.9050.9010.73712.50.6140.6420.6280.4646.250.3780.3640.3710.2073.120.2520.2490.2510.08700.1610.1660.164 | ||||||
货期：3-5 working days
×
