首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠肾胺酶(RNLS/ MAO-C)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C10orf59含量。大鼠肾胺酶（RNLS/MAO-C）是一种黄素蛋白，主要在肾脏表达，能够降解儿茶酚胺，具有改善心功能、降压和保护心肌的作用。近年来研究发现，RNLS与糖尿病及其心脑血管并发症的发生发展密切相关，其基因多态性与糖尿病的遗传易感性有关，并在糖尿病肾脏疾病、高血压、脑卒中等并发症中发挥重要作用。试剂盒检测范围为62.5 ng/mL-4000 ng/mL，适用于基础医学研究，如探索高血压、糖尿病肾病等动物模型中肾胺酶的表达调控机制，或用于药理学研究评估药物对肾脏氧化应激通路的影响。本产品仅限科学研究使用，不涉及任何临床诊断用途。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Rnls ELISA Kit; Renalase ELISA Kit; EC 1.6.3.5 ELISA Kit; Monoamine oxidase-C ELISA Kit; MAO-C ELISA Kit; alpha-NAD(P)H oxidase/anomerase ELISA Kit
缩写：C10orf59
Uniprot No.：Q5U2W9
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 ng/mL-4000 ng/mL
灵敏度：15.6 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat RNLS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
| 1:2 | Average % | 106 | |||||
| Range % | 101-113 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 90-98 | ||||||
| 1:8 | Average % | 89 | |||||
| Range % | 84-93 | ||||||
回收率：
| The recovery of rat RNLS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 87 | 82-91 | |||||
| EDTA plasma (n=4) | 95 | 90-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected40002.2042.1322.1681.97220001.6191.5241.5721.37610001.0020.9480.9750.7795000.6280.6630.6460.4502500.4300.4070.4190.2231250.2950.2810.2880.09262.50.2410.2500.2460.05000.1930.1990.196 | |||||||
货期：3-5 working days
×
