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产品描述:猪钙卫蛋白(CALP)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的CALP含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL,适用于动物疾病模型研究(如肠道炎症、感染性疾病)、免疫机制探索或药物开发中炎症标志物的动态监测。适用于兽医病理学、比较医学及畜牧养殖领域的科研分析。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
缩写:CALP
种属:Sus scrofa (Pig)
样本类型:serum, plasma, tissue homogenates
检测范围:0.312 ng/mL-20 ng/mL
灵敏度:0.078 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Others
测定原理:quantitative
测定方法:Competitive
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of pig CALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:100 | Average % | 96 | |||||
Range % | 91-102 | ||||||
1:200 | Average % | 85 | |||||
Range % | 81-89 | ||||||
1:400 | Average % | 103 | |||||
Range % | 98-107 | ||||||
1:800 | Average % | 87 | |||||
Range % | 83-92 |
回收率:
The recovery of pig CALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 94 | 88-97 | |||||
EDTA plasma (n=4) | 106 | 102-108 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
ng/mlOD1OD2Average 200.0990.1010.100 100.1600.1680.164 50.2410.2550.248 2.50.3650.3730.369 1.250.5440.5560.550 0.6250.7130.7550.734 0.3120.9841.0441.014 01.7651.8341.800 |
货期:3-5 working days
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