| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NPAP60; NPAP60L |
| Entrez GeneID | 10762 |
| WB Predicted band size | Calculated MW: 50 kDa; Observed MW: 50 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human NUP50 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于NUP50抗体的3篇参考文献的简要概括(基于公开研究内容整理，非真实文献)：
1. **文献名称**：*NUP50 regulates nuclear import through its interaction with the nuclear pore complex*
**作者**：Smith J, et al.
**摘要**：研究利用NUP50特异性抗体，通过免疫荧光和免疫沉淀技术，揭示了NUP50在核质运输中的关键作用，证明其通过与Importin-α的相互作用调控靶蛋白的核定位。
2. **文献名称**：*NUP50 dysfunction disrupts chromatin organization and gene expression*
**作者**：Lee S, et al.
**摘要**：通过NUP50抗体进行ChIP-seq和细胞成像，发现NUP50缺失导致染色质结构异常，影响基因转录调控，提示其在表观遗传学中的潜在功能。
3. **文献名称**：*NUP50 antibody validation for diagnostic applications in ovarian cancer*
**作者**：Wang Y, et al.
**摘要**：研究验证了NUP50抗体在临床样本中的特异性，发现NUP50在卵巢癌组织中高表达，可能作为预后标志物，为靶向治疗提供新方向。
如需具体文献，建议在PubMed或Google Scholar中检索关键词“NUP50 antibody”或“NUP50 function”获取最新研究。
The NUP50 antibody is a research tool targeting nucleoporin 50 (NUP50), a key component of the nuclear pore complex (NPC) that regulates nucleocytoplasmic transport. NUP50. also known as NPAP60. is part of the nuclear basket structure, interacting with proteins like Importin-α and NUP153 to facilitate mRNA export and protein import. It plays roles in cell cycle progression, chromatin organization, and gene expression. Dysregulation of NUP50 is linked to cancers (e.g., breast, liver) and developmental disorders.
NUP50 antibodies are used to study its localization, expression, and interactions via techniques like Western blot, immunofluorescence, and immunoprecipitation. They help elucidate NPC dynamics, mitotic NPC disassembly/reassembly, and disease mechanisms. Commercial NUP50 antibodies are typically raised in rabbits or mice, validated for specificity and cross-reactivity. Recent studies highlight its potential as a biomarker for cancer prognosis.
Limitations include variability in antibody performance across species or experimental conditions. Researchers must optimize protocols and verify results with knockdown/knockout controls. Ongoing work aims to clarify NUP50's regulatory networks and therapeutic targeting opportunities.
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