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产品描述：绵羊促肾上腺皮质激素(ACTH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的ACTH含量。促肾上腺皮质激素（ACTH）是腺垂体分泌的多肽激素。其它对维持肾上腺正常形态和功能有重要作用。研究机制上，ACTH作用于肾上腺皮质束状带，刺激糖皮质激素合成与分泌，在应激反应等生理过程中发挥关键调节作用。试剂盒检测范围为0.097 pg/mL-400 pg/mL，适用于绵羊等反刍动物应激状态评估、肾上腺功能研究、药理实验中激素水平动态监测等科研领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：ACTH
种属：Ovis aries (Sheep)
样本类型：serum, plasma
检测范围：0.097 pg/mL-400 pg/mL
灵敏度：0.097 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 82-89 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 88-95 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 83-95 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 95-105 | |||||
回收率：
| The recovery of sheep ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 94-103 | ||||
| EDTA plasma (n=4) | 89 | 87-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 4000.1750.1770.176 1000.2540.2680.261 250.3350.3530.344 6.250.5820.5980.590 1.560.9130.9340.924 0.391.4611.4981.480 0.0971.7511.6591.705 02.0311.9571.994 | ||||||
货期：3-5 working days
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