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产品描述：猪I型胶原(COLI)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的COLI含量。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于动物模型研究、组织工程开发、骨骼发育机制探索以及胶原代谢相关疾病的病理机制分析等科研场景，为研究猪源性生物材料或疾病模型提供可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：collagen type 1 (COL1),_x000D_ collagen type I (COLI)
缩写：COLI
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig COLⅠ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 88 | |||||
| Range % | 86-91 | ||||||
| 1:2 | Average % | 89 | |||||
| Range % | 81-95 | ||||||
| 1:4 | Average % | 98 | |||||
| Range % | 93-104 | ||||||
| 1:8 | Average % | 102 | |||||
| Range % | 98-106 | ||||||
回收率：
| The recovery of pig COLⅠ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 104 | 94-109 | |||||
| EDTA plasma (n=4) | 92 | 88-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 50002.6212.7312.6762.501 25002.1552.1772.1661.991 12501.5031.4551.4791.304 6250.9830.9300.9570.782 3120.6720.6960.6840.509 1560.4510.4440.4480.273 780.3150.3290.3220.147 00.1740.1760.175 | |||||||
货期：3-5 working days
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