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产品描述：犬白介素12(IL-12/P70)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IL12/P70含量。IL12/P70是一种重要的靶点。它在免疫调节中起关键作用，能促进Th1细胞分化、增强NK细胞活性等。研究主要围绕其参与的免疫信号通路，探索通过调控它来治疗如自身免疫性疾病、肿瘤等，有望为相关疾病治疗带来新策略。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于犬类免疫机制研究，包括炎症性疾病模型建立、疫苗免疫效果评估或感染后细胞因子动态监测等科研领域，可为动物免疫学、传染病学及比较医学研究提供可靠的实验数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL12/P70, Interleukin-12/P70
缩写：IL-12/P70
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 91-105 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 84-93 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 91-106 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of dog IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-96 | ||||
| EDTA plasma (n=4) | 97 | 92-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.9122.9452.9292.8295002.5462.5782.5622.4622501.7801.8061.7931.6931251.1271.2261.1771.07762.50.5460.5760.5610.46131.20.2590.2690.2640.16415.60.1680.1760.1720.07200.0950.1050.100 | ||||||
货期：3-5 working days
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