| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | IRF4; MUM1; Interferon regulatory factor 4; IRF-4; Lymphocyte-specific interferon regulatory factor; LSIRF; Multiple myeloma oncogene 1; NF-EM5 |
| Entrez GeneID | 3662 |
| WB Predicted band size | Calculated MW: 52 kDa; Observed MW: 52 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human MUM1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
1. **"IRF4 regulates the ratio of T helper cell subtypes by controlling the balance between opposing master regulators"**
- **作者**: Sciammas et al.
- **摘要**: 研究IRF4在调控CD4+ T细胞亚型分化中的作用，利用IRF4抗体进行ChIP-seq和Western blot实验，揭示其通过平衡不同转录因子影响Th1/Th2/Th17细胞比例。
2. **"IRF4 addiction in multiple myeloma: a pivotal therapeutic target"**
- **作者**: Shaffer et al.
- **摘要**: 分析IRF4在多发性骨髓瘤中的致癌依赖性，通过IRF4抗体染色质免疫沉淀(ChIP)发现其直接调控癌基因表达，提出靶向IRF4的治疗策略。
3. **"Requirement for the transcription factor IRF4 in B lymphocyte development"**
- **作者**: Mittrücker et al.
- **摘要**: 利用IRF4敲除小鼠和特异性抗体检测，证明IRF4对B细胞成熟和类别转换重组至关重要，揭示其在抗体多样性生成中的关键作用。
4. **"IRF4 regulates IL-17 and IL-10 expression in Th17 cells"**
- **作者**: Glasmacher et al.
- **摘要**: 通过IRF4抗体介导的流式细胞术和基因沉默，阐明IRF4在Th17细胞中协调促炎因子IL-17与抗炎因子IL-10表达的分子机制。
Interferon Regulatory Factor 4 (IRF4) is a transcription factor belonging to the IRF family, primarily involved in regulating immune cell development, differentiation, and function. It plays critical roles in lymphoid and myeloid lineages, including T-cell polarization, B-cell maturation, plasma cell differentiation, and dendritic cell activation. Dysregulation of IRF4 is linked to immune disorders and cancers, particularly hematologic malignancies like multiple myeloma, lymphoma, and acute myeloid leukemia.
IRF4 antibodies are essential tools for studying its expression, localization, and activity in both normal and pathological contexts. These antibodies enable detection of IRF4 protein via techniques such as Western blotting, immunohistochemistry (IHC), flow cytometry, and immunofluorescence (IF). They are also used in chromatin immunoprecipitation (ChIP) assays to identify IRF4-binding genomic regions.
Structurally, IRF4 contains an N-terminal DNA-binding domain and a C-terminal regulatory domain. Antibodies targeting specific epitopes can differentiate between isoforms or post-translational modifications, aiding functional studies. High specificity is crucial, as IRF4 shares homology with other IRF family members. Validated antibodies often undergo rigorous testing in knockout models or siRNA-treated cells to confirm selectivity.
In research, IRF4 antibodies help elucidate mechanisms in autoimmunity, infection responses, and oncogenesis. Clinically, they serve as diagnostic markers for certain cancers and potential therapeutic targets. Commercial IRF4 antibodies are available as monoclonal or polyclonal variants, optimized for diverse applications. Their utility in both basic and translational research underscores IRF4's significance in immunology and oncology.
×
