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产品描述：马铁调素(HAMP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HAMP含量。试剂盒检测范围为7.8 ng/mL-500 ng/mL，适用于科研领域中对铁代谢调控机制的研究，如探究贫血、铁代谢紊乱疾病的分子机制，评估动物模型或细胞实验中铁调素表达水平的变化，以及相关药物干预对铁稳态影响的体外分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HEPC, HEPCIDIN, HFE2B, LEAP-1, LEAP1, PLTR, liver-expressed antimicrobial peptide|putative liver tumor regressor
缩写：HAMP
种属：Equus caballus (Horse)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 ng/mL-500 ng/mL
灵敏度：1.95 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of horse HAMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 91 | |||||
| Range % | 86-95 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 97-107 | ||||||
| 1:4 | Average % | 91 | |||||
| Range % | 85-97 | ||||||
| 1:8 | Average % | 97 | |||||
| Range % | 91-103 | ||||||
回收率：
| The recovery of horse HAMP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 89-98 | |||||
| EDTA plasma (n=4) | 97 | 90-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 5002.5562.5302.5432.441 2502.0652.1002.0831.981 1251.6211.5691.5951.493 62.51.0781.0981.0880.986 31.20.6760.6980.6870.585 15.60.3770.3980.3880.286 7.80.2300.2440.2370.135 00.1010.1020.102 | |||||||
货期：3-5 working days
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