首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人类白细胞抗原E(HLA-E)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的HLA-E含量。HLA - E是重要免疫相关靶点。其作为非经典MHCⅠ类分子，在免疫调节中起关键作用。研究机制方面，它能与NK细胞等表面受体相互作用，抑制免疫细胞活性，影响免疫逃逸。目前围绕它开展免疫治疗研究，以打破肿瘤免疫逃逸，增强机体抗肿瘤免疫。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品可为研究HLA-E在免疫调控机制、病原体感染宿主互作、肿瘤微环境免疫抑制效应等领域的表达动态提供可靠工具，尤其适用于探索跨膜蛋白在细胞表面呈现与可溶态形式的功能差异，为免疫相关疾病的基础研究及药物开发提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HLA-E ELISA Kit; HLA-6.2 ELISA Kit; HLAEHLA class I histocompatibility antigen ELISA Kit; alpha chain E ELISA Kit; MHC class I antigen E) [Cleaved into: Soluble HLA class I histocompatibility antigen ELISA Kit; alpha chain E ELISA Kit; sHLA-E)] ELISA Kit
缩写：HLA-E
Uniprot No.：P13747
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HLA-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 85-97 | ||||||
| 1:2 | Average % | 98 | |||||
| Range % | 90-102 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 84-99 | ||||||
| 1:8 | Average % | 103 | |||||
| Range % | 95-107 | ||||||
回收率：
| The recovery of human HLA-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 99 | 88-104 | |||||
| EDTA plasma (n=4) | 97 | 88-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 20001.9761.9431.9601.788 10001.4991.4461.4731.301 5001.0180.9871.0030.831 2500.7410.7120.7270.555 1250.4570.4350.4460.274 62.50.3350.3230.3290.157 31.250.2490.2440.2470.075 00.1750.1690.172 | |||||||
货期：3-5 working days
×
