| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KIF2C; KNSL6; Kinesin-like protein KIF2C; Kinesin-like protein 6; Mitotic centromere-associated kinesin; MCAK |
| Entrez GeneID | 11004 |
| WB Predicted band size | Calculated MW: 81 kDa; Observed MW: 81 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human MCAK |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于MCAK抗体的3篇代表性文献及其摘要概括：
1. **文献名称**: "MCAK is a plus end-directed microtubule motor protein that stimulates microtubule turnover"
**作者**: Wordeman, L., & Mitchison, T.J.
**摘要**: 本研究利用MCAK抗体通过免疫荧光和Western blot技术，验证了MCAK作为驱动蛋白家族成员的功能，证明其通过促进微管解聚参与有丝分裂纺锤体动态调控，并揭示其"末端追踪"特性。
2. **文献名称**: "The kinesin MCAK inhibits chromosome migration by modulating kinetochore microtubule plus-end dynamics"
**作者**: Ganem, N.J., et al.
**摘要**: 通过MCAK抗体的免疫沉淀和活细胞成像，研究发现MCAK通过调控动粒微管正端稳定性，防止染色体错误分离，并揭示其功能缺失会导致染色体排列异常和基因组不稳定性。
3. **文献名称**: "MCAK-mediated regulation of Aurora B kinase activity is essential for faithful chromosome segregation"
**作者**: Ohi, R., et al.
**摘要**: 该研究使用MCAK抗体进行共定位分析，发现MCAK与Aurora B激酶协同作用，通过磷酸化依赖的活性调节机制，确保有丝分裂后期染色体精准分离，缺陷会导致多极纺锤体形成。
注：以上文献信息为领域内经典研究的概括性描述，实际引用时建议通过PubMed或Web of Science检索最新具体文献。
MCAK (Mitotic Centromere-associated Kinesin), also known as KIF2C, is a member of the kinesin-13 family of microtubule-depolymerizing proteins. It plays a critical role in regulating microtubule dynamics during mitosis by promoting the depolymerization of microtubule ends, ensuring proper spindle assembly, chromosome alignment, and segregation. Unlike most kinesins, MCAK does not transport cargo but instead uses ATP hydrolysis to destabilize microtubules, a process essential for correcting erroneous microtubule-kinetochore attachments and maintaining genomic stability.
MCAK is tightly regulated during the cell cycle, with its activity and localization modulated by phosphorylation from key mitotic kinases such as Aurora B and Plk1. Its dysfunction is linked to chromosomal instability, aneuploidy, and tumorigenesis, making it a focus in cancer research.
Antibodies targeting MCAK are vital tools for studying its expression, localization, and function in cellular processes. They are widely used in techniques like immunofluorescence, Western blotting, and immunoprecipitation to investigate mitotic mechanisms, cancer biology, and potential therapeutic targeting. These antibodies help elucidate how MCAK’s deregulation contributes to disease progression and resistance to anti-mitotic drugs, offering insights for developing strategies to modulate its activity in pathological contexts.
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