| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EXP; EXP35; EXP40; EXP42; MBNL; MBNL1 |
| Entrez GeneID | 4154 |
| WB Predicted band size | Calculated MW: 42 kDa; Observed MW: 42 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human MBNL1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与MBNL1抗体相关的参考文献及其简要摘要：
1. **文献名称**: "Developmental expression of mouse muscleblind-like genes"
**作者**: Ho TH et al. (2005)
**摘要**: 本研究利用特异性MBNL1抗体，通过Western blot和免疫组化技术，系统分析了小鼠胚胎及成体组织中MBNL1蛋白的时空表达模式，发现其在肌肉和神经组织中高表达，并参与发育调控。
2. **文献名称**: "MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing"
**作者**: Goers ES et al. (2010)
**摘要**: 通过免疫沉淀(IP)结合RNA测序技术，验证了MBNL1抗体在捕获RNA-蛋白复合物中的特异性，揭示了MBNL1通过结合特定GC富集区调控选择性剪接的分子机制。
3. **文献名称**: "Antibody characterization of muscleblind-like proteins in myotonic dystrophy"
**作者**: Fardaei M et al. (2002)
**摘要**: 首次报道了针对MBNL1蛋白的多克隆抗体制备及验证，证实其在患者肌肉组织检测中的可靠性，发现强直性肌营养不良患者中MBNL1蛋白存在异常核聚集现象。
4. **文献名称**: "Cellular localization and splicing regulation of MBNL1 in mammalian development"
**作者**: Konieczny P et al. (2014)
**摘要**: 利用荧光标记的MBNL1抗体进行共聚焦显微镜分析，揭示了该蛋白在细胞质与细胞核间的动态分布，及其剪接活性受亚细胞定位调控的生理机制。
注：以上文献信息为示例性质，实际引用前请核对原文准确性。建议通过PubMed或Google Scholar以“MBNL1 antibody”+“validation/application”等关键词检索最新研究。
The MBNL1 antibody is a key tool for studying the Muscleblind-like protein 1 (MBNL1), an RNA-binding protein critical for regulating alternative splicing, mRNA localization, and stability in mammalian cells. MBNL1 belongs to the Muscleblind-like family (MBNL1-4), which plays a vital role in developmental transitions, including muscle and neural maturation. Dysregulation of MBNL1 is strongly linked to myotonic dystrophy (DM), a genetic disorder caused by expanded CTG/CUG repeats in non-coding regions of the *DMPK* or *CNBP* genes. These repeats sequester MBNL1 into nuclear foci, depleting its availability for normal RNA processing, leading to mis-splicing events and disease symptoms like myotonia and muscle wasting.
Antibodies targeting MBNL1 are widely used in research to detect protein expression, subcellular localization (e.g., nuclear vs. cytoplasmic distribution), and interactions in cell and animal models. They are essential for Western blotting, immunofluorescence, and immunohistochemistry to assess MBNL1 dynamics in healthy versus diseased tissues, particularly in DM studies. Commercially available MBNL1 antibodies are typically raised against specific epitopes, such as the N-terminal or C-terminal regions, and require validation for species reactivity (human, mouse, rat) and application-specific performance. Researchers also utilize these antibodies to explore therapeutic strategies aimed at releasing MBNL1 from RNA foci or enhancing its function. Given MBNL1's role beyond DM, including in cancer and other splicing-related disorders, its antibody remains a versatile reagent in molecular and clinical research.
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