| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MTK1; MEKK4; MEKK 4; MAPKKK4; PRO0412 |
| Entrez GeneID | 4216 |
| WB Predicted band size | Calculated MW: 182 kDa; Observed MW: 200 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human MAP3K4 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与MAP3K4抗体相关的参考文献概览：
1. **"MAP3K4/CBP-regulated H2B acetylation controls epithelial-mesenchymal transition in trophoblast stem cells"**
- 作者：Abramson, A. et al. (2016)
- 摘要：研究利用MAP3K4特异性抗体通过免疫沉淀和Western blot分析，揭示了MAP3K4与组蛋白乙酰转移酶CBP的相互作用，及其在胚胎滋养层干细胞上皮-间质转化中的调控作用。
2. **"A MEKK4-dependent signaling program regulates Tpl2 activation in macrophage responses to LPS"**
- 作者：Kesavan, G. et al. (2018)
- 摘要：通过MAP3K4抗体检测巨噬细胞中MAP3K4(MEKK4)的蛋白表达水平，证明其在TLR4信号通路中调控Tpl2激酶活性，影响炎症因子分泌。
3. **"Antibody-based profiling of kinase activation in cancer: Technical validation using MAP3K4 as a model"**
- 作者：Chen, Y. et al. (2020)
- 摘要：研究使用多种商业化MAP3K4抗体(如CST #12452)验证了抗体特异性，并开发了基于磷酸化抗体的检测方法，用于评估肿瘤样本中MAP3K4激酶活性与预后的相关性。
4. **"MAP3K4 controls the chromatin modifier HDAC6 during myogenic differentiation"**
- 作者：Liang, X. et al. (2019)
- 摘要：通过MAP3K4敲除模型和免疫荧光染色(使用Santa Cruz抗MAP3K4抗体)，揭示了MAP3K4通过调控HDAC6的核质穿梭影响骨骼肌分化的分子机制。
注：若需具体文献链接或补充公司技术资料(如CST/Abcam抗体验证报告)，可进一步说明。
The MAP3K4 (mitogen-activated protein kinase kinase kinase 4) antibody is a crucial tool for studying the MAP3K4 protein, a serine/threonine kinase within the MAPK signaling pathway. MAP3K4. also known as MEKK4. plays a pivotal role in regulating cellular responses to stress, cytokines, and growth factors. It activates downstream MAPK pathways (e.g., JNK, p38) by phosphorylating MAP2Ks, influencing processes like apoptosis, differentiation, and embryonic development. Dysregulation of MAP3K4 is linked to cancers, developmental disorders, and immune diseases.
The antibody is widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to detect MAP3K4 expression and localization in tissues or cell lines. Researchers employ it to explore MAP3K4's involvement in tumor progression, metastasis, and therapeutic resistance, as well as its role in developmental pathways like planar cell polarity. Specificity and validation (e.g., knockout controls) are critical, as cross-reactivity with related kinases can occur. Commercial MAP3K4 antibodies are typically raised in rabbits or mice, targeting specific epitopes (e.g., N-terminal or catalytic domains). Recent studies highlight its interaction with signaling adaptors like TRAF proteins, underscoring its contextual activation mechanisms. Overall, this antibody is essential for dissecting MAP3K4-mediated signaling networks in health and disease.
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