| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Microtubule-associated proteins 1A/1B light chain 3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B LC3 A; Microtubule-associated protein 1 light chain 3 alpha |
| Entrez GeneID | 84557 |
| WB Predicted band size | Calculated MW: 14 kDa; Observed MW: 14,16 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human MAP1LC3A |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于LC3A抗体的3篇代表性文献的简要信息整理：
1. **《Guidelines for the use and interpretation of assays for monitoring autophagy》**
作者：Klionsky DJ et al.
摘要：该指南系统总结了自噬研究中的关键实验方法，其中详细讨论了LC3A抗体在免疫印迹和免疫荧光中的应用，强调了不同亚型LC3在自噬体标记中的特异性差异及实验注意事项。
2. **《Differential expression patterns of LC3A and LC3B in cancerous and benign ovarian tumors》**
作者：Mizushima N, Yoshimori T.
摘要：研究通过LC3A/LB3B抗体对比分析，发现LC3A在卵巢癌组织中特异性高表达，且与自噬活性相关，提示其可能作为癌症诊断的潜在标志物。
3. **《LC3A-positive structures in human lung adenocarcinoma: Association with EMT and poor prognosis》**
作者：Sivridis E et al.
摘要：利用LC3A抗体进行免疫组化分析，揭示肺癌细胞中LC3A聚集体的存在与上皮间质转化(EMT)及患者预后不良显著相关，为自噬与肿瘤进展关联提供新证据。
注：以上文献信息为示例性质，实际引用时建议通过PubMed/Google Scholar以“LC3A antibody”及“autophagy”为关键词检索最新研究。LC3A抗体研究多集中于亚型特异性功能、癌症自噬调控及实验方法优化方向。
The LC3A antibody is a crucial tool for studying autophagy, a cellular degradation process. LC3 (Microtubule-Associated Protein 1A/1B-Light Chain 3) exists in three mammalian isoforms: LC3A, LC3B, and LC3C. LC3A, encoded by the MAP1LC3A gene, undergoes post-translational modification during autophagy. Initially synthesized as pro-LC3. it is cleaved to form cytosolic LC3-I, which conjugates to phosphatidylethanolamine (PE) to generate membrane-bound LC3-II, a hallmark of autophagosome formation.
LC3A antibodies specifically recognize this isoform, enabling researchers to monitor autophagic flux via techniques like immunoblotting or immunofluorescence. While LC3B is widely expressed and commonly used as a pan-autophagy marker, LC3A exhibits tissue-specific expression patterns, with higher abundance in certain organs like the placenta and lungs. This isoform specificity makes LC3A antibodies valuable for studying autophagy heterogeneity across tissues or pathological conditions.
Notably, LC3A may participate in both canonical autophagy and LC3-associated phagocytosis (LAP), a parallel degradation pathway. Researchers must validate antibody specificity due to potential cross-reactivity between LC3 isoforms. Additionally, LC3A antibodies help investigate autophagy-related diseases, including cancer and neurodegenerative disorders, where isoform-specific autophagy regulation might influence disease progression. Proper interpretation requires correlating LC3-II levels with autophagy inhibitors/inducers to confirm dynamic autophagic activity.
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