首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠细胞凋亡调节因子BAX(BAX)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的BAX含量。BAX是一种重要的促凋亡蛋白。其背景在于它属于Bcl-2蛋白家族成员。研究机制方面，在细胞受到凋亡刺激时，BAX会从胞质转位到线粒体膜上，促使线粒体膜通透性改变，释放细胞色素C等促凋亡物质，进而激活凋亡级联反应。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于科研场景中细胞凋亡机制研究、药物诱导的凋亡效应评估或疾病模型（如缺血再灌注损伤、癌症）中BAX表达水平的动态监测，为探索凋亡相关信号通路提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Bax; Apoptosis regulator BAX
缩写：BAX
Uniprot No.：Q63690
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat BAX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 85-92 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 87-99 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 82-98 | |||||
回收率：
| The recovery of rat BAX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-97 | ||||
| EDTA plasma (n=4) | 97 | 93-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 40002.0911.9952.0431.844 20001.7511.7761.7641.565 10001.3681.4321.4001.201 5000.9850.9720.9790.780 2500.5350.5440.5400.341 1250.3480.3520.3500.151 62.50.2440.2370.2410.042 00.2010.1970.199 | ||||||
货期：3-5 working days
×
