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Mouse angiotensin Ⅱ(ANG-Ⅱ) ELISA Kit

  • 中文名: 小鼠血管紧张素Ⅱ(ANG-Ⅱ)ELISA Kit
货号: IPD-E04495m
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    小鼠血管紧张素Ⅱ(ANG-Ⅱ)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测血清、血浆样本中的ANG-Ⅱ含量。ANG-Ⅱ即血管紧张素Ⅱ,在人体血压调节、体液平衡等生理过程中扮演关键角色。其过度激活与高血压、心血管疾病等多种病症相关。研究主要聚焦于其信号传导通路、与受体相互作用机制,为研发针对性药物以防治相关疾病提供理论依据。试剂盒检测范围为15.6 pg/ml-1000 pg/ml.,适用于心血管疾病动物模型研究、降压药物作用机制探索、内分泌调控实验等科研领域,为小鼠血清或血浆样本中ANG - Ⅱ的动态监测提供可靠工具本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    angiotensin Ⅱ, angiotensin 2, ANG2
  • 缩写:
    ANG-Ⅱ
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma
  • 检测范围:
    15.6 pg/ml-1000 pg/ml.
  • 灵敏度:
    7.8 pg/ml.
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cardiovascular
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<12%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<15%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANG-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    88


    Range %

    83-94


    1:2

    Average %

    91


    Range %

    85-96


    1:4

    Average %

    90


    Range %

    84-98


    1:8

    Average %

    93


    Range %

    86-99


  • 回收率:

    The recovery of mouse ANG-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    91

    84-98


    EDTA plasma (n=4)

    87

    81-95


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average




    1000

    0.161

    0.166

    0.164




    500

    0.185

    0.192

    0.189




    250

    0.228

    0.237

    0.233




    125

    0.334

    0.324

    0.329




    62.5

    0.568

    0.576

    0.572




    31.25

    0.902

    0.891

    0.897




    15.6

    1.208

    1.217

    1.212




    0

    2.176

    2.223

    2.199





  • 货期:
    3-5 working days

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