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Intra-assay Precision (Precision within an assay): CV%<12% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<15% | ||||||
Three samples of known concentration were tested in twenty assays to assess. |
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANG-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 88 | ||||
Range % | 83-94 | |||||
1:2 | Average % | 91 | ||||
Range % | 85-96 | |||||
1:4 | Average % | 90 | ||||
Range % | 84-98 | |||||
1:8 | Average % | 93 | ||||
Range % | 86-99 |
The recovery of mouse ANG-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 91 | 84-98 | ||||
EDTA plasma (n=4) | 87 | 81-95 |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
pg/ml | OD1 | OD2 | Average | |||
1000 | 0.161 | 0.166 | 0.164 | |||
500 | 0.185 | 0.192 | 0.189 | |||
250 | 0.228 | 0.237 | 0.233 | |||
125 | 0.334 | 0.324 | 0.329 | |||
62.5 | 0.568 | 0.576 | 0.572 | |||
31.25 | 0.902 | 0.891 | 0.897 | |||
15.6 | 1.208 | 1.217 | 1.212 | |||
0 | 2.176 | 2.223 | 2.199 |
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