首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠神经肽Y(NPY)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的NPY含量。NPY即神经肽Y，在人体广泛分布。它参与调节多种生理功能如食欲、血压等。研究机制方面，NPY主要通过与不同受体亚型结合发挥作用，其信号通路与能量代谢、心血管功能等密切相关，是神经生物学等领域的重要研究靶点。试剂盒检测范围为1.87 pg/mL-120 pg/mL，适用于神经生物学、代谢调控及药物开发等科研领域的研究；尤其适用于动物模型中的NPY动态监测、神经递质通路研究以及体外实验中细胞因子分泌水平的评估，为探索NPY在生理及疾病机制中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Npy ELISA Kit; Pro-neuropeptide Y [Cleaved into: Neuropeptide Y ELISA Kit; Neuropeptide tyrosine ELISA Kit; NPY); C-flanking peptide of NPY ELISA Kit; CPON)] ELISA Kit
缩写：NPY
Uniprot No.：P57774
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：1.87 pg/mL-120 pg/mL
灵敏度：0.46 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse NPY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 84-96 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 89-96 | |||||
回收率：
| The recovery of mouse NPY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 98-106 | ||||
| EDTA plasma (n=4) | 88 | 82-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected1202.2042.1032.1542.013601.5551.4541.5051.364300.8830.8620.8730.732150.4950.5060.5010.3607.50.3270.3160.3220.1813.750.2340.2450.2400.0991.870.1790.1750.1770.03600.1410.1400.141 | ||||||
货期：3-5 working days
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