首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠线粒体呼吸链复合物I酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的MRCC-I含量。试剂盒检测范围为7.8 pg/mL-500 pg/mL，本试剂盒采用高特异性配对抗体，通过标准化的预包被板与显色系统实现目标蛋白的定量分析，适用于基础研究中线粒体功能评估、疾病模型构建（如心肌缺血、阿尔茨海默症等）或药物干预后能量代谢变化的动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：MRCC-I
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MRCC-I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 96 | |||||
| Range % | 84-100 | ||||||
| 1:2 | Average % | 100 | |||||
| Range % | 94-103 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 84-98 | ||||||
| 1:8 | Average % | 100 | |||||
| Range % | 93-103 | ||||||
回收率：
| The recovery of mouse MRCC-I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 84-97 | |||||
| EDTA plasma (n=4) | 99 | 91-103 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected5002.1872.0992.1431.9722501.5491.4761.5131.3421251.0981.0441.0710.90062.50.8120.7890.8010.63031.20.5750.5450.5600.38915.60.4160.4230.4200.2497.80.2930.2810.2870.11600.1690.1730.171 | |||||||
货期：3-5 working days
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