首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠嗜酸性粒细胞阳离子蛋白(ECP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的ECP含量。ECP即嗜酸性粒细胞阳离子蛋白，在过敏等多种免疫相关疾病背景下发挥作用。研究机制方面，它具有细胞毒性，可破坏寄生虫、肿瘤细胞等，还能趋化炎症细胞，参与炎症反应的调控，在疾病发生发展中扮演重要角色，是潜在治疗靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于科研场景中评估疾病模型（如哮喘、过敏或寄生虫感染）的嗜酸性粒细胞激活程度，支持多种样本类型的平行检测，为研究 Th2 型免疫反应、组织炎症机制及药物干预效果提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：ECP
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ECP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-98 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-106 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 88-99 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 83-95 | |||||
回收率：
| The recovery of mouse ECP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-97 | ||||
| EDTA plasma (n=4) | 95 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.5462.6522.5992.43552.0742.0762.0751.9112.51.4131.5041.4591.2951.250.6760.7350.7060.5420.6250.4860.5240.5050.3410.3120.3840.3590.3720.2080.1560.2510.2590.2550.09100.1630.1650.164 | ||||||
货期：3-5 working days
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