首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠基质金属蛋白酶7(MMP7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的MMP7含量。MMP7即基质金属蛋白酶7，在细胞外基质降解、肿瘤发生发展等过程中发挥重要作用。其研究机制主要聚焦于它对基底膜和细胞外基质成分的降解，促进肿瘤细胞侵袭转移，还参与炎症反应、血管生成等，是肿瘤等疾病的潜在治疗靶点。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于多种科研场景，如肿瘤微环境研究中MMP7的表达水平分析、炎症性疾病模型中的蛋白酶活性评估，或组织修复机制探索实验中细胞分泌动态监测。其宽泛的样本兼容性支持研究人员针对不同实验体系（如体外细胞培养分泌检测、动物模型组织提取物分析等）开展定量研究，为探究MMP7在疾病发生发展中的调控作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Mmp7Matrilysin ELISA Kit; EC 3.4.24.23 ELISA Kit; Matrin ELISA Kit; Matrix metalloproteinase-7 ELISA Kit; MMP-7 ELISA Kit; Pump-1 protease ELISA Kit; Uterine metalloproteinase ELISA Kit
缩写：MMP7
Uniprot No.：Q10738
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 90-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 98-104 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 95-102 | |||||
回收率：
| The recovery of mouse MMP7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 92-96 | ||||
| EDTA plasma (n=4) | 96 | 92-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.1802.0882.1341.98110001.5481.5341.5411.3885000.8820.8990.8910.7382500.5110.4890.5000.3471250.3380.3310.3350.18262.50.2560.2480.2520.09931.250.2020.2110.2070.05400.1540.1520.153 | ||||||
货期：3-5 working days
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