| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CKN1; CSA; ERCC8 |
| Entrez GeneID | 1161 |
| WB Predicted band size | Calculated MW: 44 kDa; Observed MW: 44 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human ERCC8 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是关于ERCC8抗体的参考文献示例(文献信息为虚构，仅供示例参考)：
1. **文献名称**：ERCC8 Antibody Validation in DNA Repair Studies
**作者**：Smith et al.
**摘要**：本研究验证了一种高特异性ERCC8抗体在Western blot和免疫荧光中的应用，证实其在检测人类细胞中ERCC8蛋白表达及定位的可靠性，并发现ERCC8缺失导致紫外线诱导的DNA损伤修复能力下降。
2. **文献名称**：Role of ERCC8 in Cockayne Syndrome Pathogenesis
**作者**：Chen & Wang
**摘要**：通过免疫组化结合ERCC8抗体，作者发现科凯恩综合征患者成纤维细胞中ERCC8蛋白表达显著降低，提示其与患者早衰表型及神经退行性病变相关。
3. **文献名称**：ERCC8 Antibody Screening for Cancer Biomarker Discovery
**作者**：Rodriguez et al.
**摘要**：利用ERCC8抗体对多种癌症组织样本进行分析，发现ERCC8低表达与肺癌化疗耐药性相关，提示其可能作为预测治疗反应的潜在生物标志物。
4. **文献名称**：ERCC8 Knockout Mouse Model Characterization
**作者**：Kimura et al.
**摘要**：通过ERCC8特异性抗体检测基因敲除小鼠模型，发现ERCC8缺失导致小鼠胚胎发育异常及DNA修复缺陷，进一步支持其在核苷酸切除修复中的核心作用。
---
注：以上内容为模拟生成，实际文献需通过PubMed/Google Scholar等平台检索关键词(如"ERCC8 antibody" "Cockayne syndrome" "DNA repair")。
The ERCC8 antibody targets the ERCC8 protein (also known as CSA), a critical component of the transcription-coupled nucleotide excision repair (TC-NER) pathway, which repairs DNA damage caused by UV-induced lesions or chemical adducts. ERCC8 forms a complex with other DNA repair proteins, including CSB (ERCC6), to recognize RNA polymerase II stalled at DNA lesions, facilitating recruitment of repair factors. Mutations in the ERCC8 gene are linked to Cockayne syndrome (CS), a rare autosomal recessive disorder characterized by growth failure, neurological dysfunction, and photosensitivity.
ERCC8 antibodies are widely used in research to study DNA repair mechanisms, protein interactions, and disease pathogenesis. They enable detection of ERCC8 expression via techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies also aid in investigating CSA's role in aging, neurodegeneration, and cancer, as TC-NER deficiency contributes to genomic instability. Validation of ERCC8 antibodies often involves knockout cell lines to confirm specificity. Commercially available ERCC8 antibodies are typically produced in hosts like rabbits or mice, with formats including monoclonal or polyclonal variants. Their applications extend to elucidating molecular pathways in CS patient-derived cells and exploring therapeutic strategies targeting DNA repair deficiencies.
×
