首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠多肽YY(PYY)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的PYY含量。试剂盒检测范围为94 pg/mL-6000 pg/mL，适用于胃肠道功能研究、代谢性疾病模型评估、药物干预效果分析等科研场景，为探索能量代谢调控机制及靶向治疗研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Pyy ELISA Kit; Peptide YY ELISA Kit; PYY ELISA Kit; Peptide tyrosine tyrosine) [Cleaved into: Peptide YY(3-36 ELISA Kit; PYY-II)] ELISA Kit
缩写：PYY
Uniprot No.：Q9EPS2
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：94 pg/mL-6000 pg/mL
灵敏度：23.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PYY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 104 | ||||
| Range % | 100-108 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 93-99 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 95-105 | |||||
回收率：
| The recovery of mouse PYY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 94-102 | ||||
| EDTA plasma (n=4) | 86 | 83-89 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected60002.3032.3692.3362.22430001.6061.6581.6321.52015000.9910.9680.9800.8687500.6060.5690.5880.4763750.3760.3980.3870.275187.50.2930.2900.2920.180940.1890.1920.1910.07900.1110.1130.112 | ||||||
货期：3-5 working days
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