首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠骨形成蛋白6(BMP-6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的BMP6含量。BMP6 即骨形态发生蛋白 6，属于 TGF - β超家族成员。它在骨骼发育、铁代谢调节等生理过程中发挥重要作用。研究机制上，其可结合特定受体，激活下游信号通路，调控相关基因表达，进而影响细胞的增殖、分化等活动。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品广泛应用于细胞模型、动物实验或病理机制研究中BMP - 6的动态监测，例如探究成骨分化、铁稳态调控或组织再生过程中的分子机制，为骨骼发育、代谢调控及组织修复等基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Bmp6 ELISA kit; Bmp-6 ELISA kit; Vgr1Bone morphogenetic protein 6 ELISA kit; BMP-6 ELISA kit; VG-1-related protein ELISA kit; VGR-1 ELISA kit
缩写：BMP6
Uniprot No.：P20722
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse BMP-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 82-101 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 93-107 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-105 | |||||
回收率：
| The recovery of mouse BMP-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 95-105 | ||||
| EDTA plasma (n=4) | 102 | 96-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.6162.5822.5992.41410002.2152.2342.2252.0405001.8011.8341.8181.6332501.3671.3301.3491.1641250.7680.7430.7560.57162.50.4690.4420.4560.27131.250.2720.2920.2820.09700.1840.1860.185 | ||||||
货期：3-5 working days
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