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产品描述：人左右不对称发育因子2(LEFTY2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的LEFTY2含量。试剂盒检测范围为7.8 pg/mL-500 pg/mL，该产品适用于基础科研领域，例如探究LEFTY2在胚胎发育异常、纤维化疾病或肿瘤发生发展中的分子机制，也可用于评估细胞模型或动物模型中LEFTY2的表达水平变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：EBAF ELISA Kit; Endometrial bleeding associated factor (left right determination factor A transforming growth factor beta superfamily) ELISA Kit; Endometrial bleeding associated factor ELISA Kit; Endometrial bleeding-associated factor ELISA Kit; Left right determination factor 2 ELISA Kit; Left right determination factor A ELISA Kit; Left-right determination factor 2 ELISA Kit; Left-right determination factor A ELISA Kit; LEFTA ELISA Kit; LEFTY 2 ELISA Kit; LEFTY2 ELISA Kit; LEFTYA ELISA Kit; LFTY2 transforming growth factor; beta -4 ELISA Kit; LFTY2; mouse; homolog of ELISA Kit; LFTY2_HUMAN ELISA Kit; MGC46222 ELISA Kit; Protein lefty 2 ELISA Kit; Protein lefty A ELISA Kit; Protein lefty-2 ELISA Kit; Protein lefty-A ELISA Kit; Protein lefty2 ELISA Kit; PSEC0024 ELISA Kit; TGF beta 4 ELISA Kit; TGF-beta-4 ELISA Kit; TGFB4 ELISA Kit; Transforming growth factor beta 4 ELISA Kit; Transforming growth factor beta-4 ELISA Kit
缩写：LEFTY2
Uniprot No.：O00292
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LEFTY2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 91 | |||||
| Range % | 86-95 | ||||||
| 1:200 | Average % | 102 | |||||
| Range % | 97-107 | ||||||
| 1:400 | Average % | 91 | |||||
| Range % | 85-97 | ||||||
| 1:800 | Average % | 97 | |||||
| Range % | 91-103 | ||||||
回收率：
| The recovery of human LEFTY2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 89-98 | |||||
| EDTA plasma (n=4) | 97 | 90-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected5002.8112.8362.8242.7442502.0382.0002.0191.9391251.3311.3681.3501.27062.50.8040.7520.7780.69831.20.4730.4980.4860.40615.60.2780.2810.2800.2007.80.1730.1690.1710.09100.0790.0810.080 | |||||||
货期：3-5 working days
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